FlyBase Allele Report: Dmel\Rab35[EYFP]

General Information

Symbol

Dmel\Rab35^EYFP

Species

D. melanogaster

Name

FlyBase ID

FBal0314212

Feature type

allele

Associated gene

Dmel\Rab35

Associated Insertion(s)

TI{TI}Rab35^EYFP

Carried in Construct

Also Known As

Yrab35

Key Links

Allele class

Mutagen

ends-in gene targeting

Nature of the Allele

Allele class

Mutagen

ends-in gene targeting

(Dunst et al., 2015)

Progenitor genotype

Associated Insertion(s)

TI{TI}Rab35^EYFP

Cytology

Description

The predicted start codon is replaced with EYFP and Tag:MYC, creating a endogenous N-terminally tagged Rab protein.

(Dunst et al., 2015)

Allele components

Component

Use(s)

Tagged with

EYFP

yellow fluorescent protein

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity, with Avic\GFP^RNAi.R.UAS, Scer\GAL4^nub.PK

(Dunst et al., 2015)

abnormal planar polarity, with Avic\GFP^{RNAi.UAS.V20.3}, Scer\GAL4^nub.PK

(Dunst et al., 2015)

viable

(Dunst et al., 2015)

Phenotype Manifest In

wing hair, with Avic\GFP^RNAi.R.UAS, Scer\GAL4^nub.PK

(Dunst et al., 2015)

wing hair, with Avic\GFP^{RNAi.UAS.V20.3}, Scer\GAL4^nub.PK

(Dunst et al., 2015)

Detailed Description

Statement

Reference

Scer\GAL4^nub.PK-mediated expression of Avic\GFP^{dsRNA.shRNA.Scer\UAS.3} or Avic\GFP^{dsRNA.R.Scer\UAS} in Rab35^EYFP homozygotes (which effectively reduces endogenous Rab35 levels) results in occasional subtle disturbances in wing hair morphology.

(Dunst et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Rab35^EYFP, Scer\GAL4^Hml.Δ, Tcs5^RNAi.UAS.462 has abnormal cell migration | P-stage phenotype

(Molina et al., 2022)

Avic\GFP^{RNAi.UAS.V20.3}, Rab23^EYFP, Rab35^EYFP, Scer\GAL4^nub.PK has abnormal planar polarity phenotype

(Dunst et al., 2015)

Avic\GFP^RNAi.R.UAS, Rab23^EYFP, Rab35^EYFP, Scer\GAL4^nub.PK has abnormal planar polarity phenotype

(Dunst et al., 2015)

Phenotype Manifest In

Other

Statement

Reference

Rab35^EYFP, Scer\GAL4^Hml.Δ, Tcs5^RNAi.UAS.462 has hemocyte | P-stage phenotype

(Molina et al., 2022)

Avic\GFP^{RNAi.UAS.V20.3}, Rab23^EYFP, Rab35^EYFP, Scer\GAL4^nub.PK has wing hair phenotype

(Dunst et al., 2015)

Avic\GFP^RNAi.R.UAS, Rab23^EYFP, Rab35^EYFP, Scer\GAL4^nub.PK has wing hair phenotype

(Dunst et al., 2015)

Avic\GFP^RNAi.R.UAS, Rab23^EYFP, Rab35^EYFP, Scer\GAL4^nub.PK has crossvein | ectopic phenotype

(Dunst et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^nub.PK-mediated expression of Avic\GFP^{dsRNA.shRNA.Scer\UAS.3} or Avic\GFP^{dsRNA.R.Scer\UAS} in Rab23^EYFP Rab35^EYFP double homozygotes (which effectively reduces endogenous Rab23 and Rab35 levels) results in additive wing hair polarity and morphology defects, plus the formation and ectopic cross veins.

(Dunst et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

62559

w¹¹¹⁸ TI{TI}Rab35^EYFP

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Rab35^EYFP

(Bellec et al., 2018)

YRab35

(Caviglia et al., 2016)

Yrab35

(Dunst et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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