FlyBase Allele Report: Dmel\bark[L200]

General Information

Symbol

Dmel\bark^L200

Species

D. melanogaster

Name

FlyBase ID

FBal0314306

Feature type

allele

Associated gene

Dmel\bark

Associated Insertion(s)

Carried in Construct

Also Known As

aka^L200

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Byri et al., 2015)

amorphic allele - molecular evidence

(Byri et al., 2015)

Mutagen

ethyl methanesulfonate

(Byri et al., 2015)

Progenitor genotype

Cytology

Description

127bp deletion.

(Luschnig, 2016.1.10)

Deletion in the third coding exon of bark resulting in a frame-shift and a premature stop codon six codons thereafter. This should truncate the protein to 587 aa after the CUB-like domain in region I of the extracellular part.

(Byri et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:3,793,915..3,794,041 [+]

Comment:

A 127 bp deletion that results in a frameshift and a premature stop codon 6 amino acids downstream.

(Luschnig, 2016.1.10)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell adhesion

(Byri et al., 2015)

decreased cell size | P-stage | somatic clone

(Esmangart de Bournonville et al., 2024)

lethal - all die during embryonic stage

(Byri et al., 2015)

Phenotype Manifest In

adherens junction | P-stage | somatic clone

(Esmangart de Bournonville et al., 2024)

basal part of cell | P-stage | somatic clone

(Esmangart de Bournonville et al., 2024)

cell-cell junction | P-stage | somatic clone

(Esmangart de Bournonville et al., 2024)

embryonic/larval tracheal section

(Byri et al., 2015)

embryonic/larval tracheal section (with Df(2L)Exel6009)

(Byri et al., 2015)

embryonic epidermis

(Wittek et al., 2020)

epithelial cell | P-stage | somatic clone

(Esmangart de Bournonville et al., 2024)

epithelial cell | somatic clone

(Daniel et al., 2018)

filamentous actin | P-stage | somatic clone

(Esmangart de Bournonville et al., 2024)

mesonotum | P-stage | somatic clone

(Esmangart de Bournonville et al., 2024)

midbody | somatic clone

(Daniel et al., 2018)

plasma membrane | somatic clone

(Daniel et al., 2018)

septate junction | somatic clone

(Daniel et al., 2018)

tricellular tight junction

(Byri et al., 2015)

tricellular tight junction | embryonic stage

(Wittek et al., 2020)

vesicle | P-stage | increased number | somatic clone

(Esmangart de Bournonville et al., 2024)

Detailed Description

Statement

Reference

bark^L200 embryonic epidermis exhibit disappearance of the low mobility of GFP::M6 at tricellular junctions.

(Wittek et al., 2020)

bark^L200 clones of dividing epithelial cells have membrane deformations, disruptions in the integrity of septate junctions and an accelerated basal displacement of the midbody. The assembly and stability of septate junctions are unaffected.

bark^L200 clones of epithelial cells have normal finger-like protrusions associated with the midbody of neighboring dividing cells.

(Daniel et al., 2018)

Mutant embryos show excessively elongated tracheal tubes. Stage 17 bark^L200 embryos also show 'cavities' in the tracheal epithelium, seen as round areas 0.5-6 mm in diameter, that form between neighbouring cells. Similar structures are evident in the epidermis.

The formation and correct geometry of tricellular junctions, but not bicellular septate junctions, is defective in bark^L200 embryos.

(Byri et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

bark^L200 has decreased cell size | somatic clone | P-stage phenotype, suppressible by crb^11A22

(Esmangart de Bournonville et al., 2024)

bark^L200 has decreased cell size | somatic clone | P-stage phenotype, suppressible by mys^JF02819/Scer\GAL4^Tub.PU

(Esmangart de Bournonville et al., 2024)

Phenotype Manifest In

Suppressed by

Statement

Reference

bark^L200 has cell-cell junction | somatic clone | P-stage phenotype, suppressible by mys^JF02819/Scer\GAL4^Tub.PU

(Esmangart de Bournonville et al., 2024)

bark^L200 has epithelial cell | somatic clone | P-stage phenotype, suppressible by mys^JF02819/Scer\GAL4^Tub.PU

(Esmangart de Bournonville et al., 2024)

bark^L200 has epithelial cell | somatic clone | P-stage phenotype, suppressible | partially by crb^11A22

(Esmangart de Bournonville et al., 2024)

NOT suppressed by

Statement

Reference

bark^L200 has cell-cell junction | somatic clone | P-stage phenotype, non-suppressible by crb^11A22

(Esmangart de Bournonville et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

bark^L200 is rescued by bark^UAS.cBa/Scer\GAL4^btl.PU

(Byri et al., 2015)

bark^L200 is rescued by Scer\GAL4^btl.PU/bark^ΔPDZB.UAS

(Byri et al., 2015)

Comments

Scer\GAL4^btl.PU-mediated expression of bark^Scer\UAS.cBa or bark^{ΔPDZB.Scer\UAS} rescues the tracheal defects of bark^L200 embryos.

(Byri et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

aka^L200

(Mira-Osuna et al., 2025, Esmangart de Bournonville et al., 2024, Schleutker and Luschnig, 2024, Isasti-Sanchez et al., 2021, Wittek et al., 2020, Byri et al., 2015)

bark^L200

(Daniel et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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