FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Nep2MI01118-GFSTF.2
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General Information
Symbol
Dmel\Nep2MI01118-GFSTF.2
Species
D. melanogaster
Name
FlyBase ID
FBal0314693
Feature type
allele
Associated gene
Dmel\Nep2
Associated Insertion(s)
Mi{PT-GFSTF.2}Nep2MI01118-GFSTF.2
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Gene Disruption Project members, 2015-)
Progenitor genotype
Mi{MIC}Nep2MI01118
(Gene Disruption Project members, 2015-)
Associated Insertion(s)
Mi{PT-GFSTF.2}Nep2MI01118-GFSTF.2
Cytology
Description

The RMCE cassette of the Mi{MIC}Nep2MI01118 insertion has been replaced with a EGFP-FlAsH-StrepII-TEV-3xFlag multi-tag cassette. The insertion is in a coding intron of Nep2 and is predicted to protein trap the gene.

(Gene Disruption Project members, 2015-)
Allele components
Component
Use(s)
Inserted element
Mi{PT-GFSTF.2}
Tagged with
EGFP
Tag:CALI(TC)
Tag:CS(TEVp)
Tag:FLAG
Tag:StrepII
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      oogenesis stage S9 -- S10
      border follicle cell
      (Manning et al., 2017)
      centripetal follicle cell
      (Manning et al., 2017)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Nep2
      (Manning et al., 2017)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Nep2MI01118-GFSTF.2
      Name Synonyms
      Secondary FlyBase IDs
        References (2)