FlyBase Allele Report: Hsap\APP[Aβ43.UAS.Tag:SS(nec).cBa]

General Information

Symbol

Hsap\APP^{Aβ43.UAS.Tag:SS(nec).cBa}

Species

H. sapiens

Name

FlyBase ID

FBal0314800

Feature type

allele

Associated gene

Hsap\APP

Associated Insertion(s)

Carried in Construct

P{UAS-Aβ43.B}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Burnouf et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-Aβ43.B}

Cytology

Description

UASt regulatory sequences drive expression of amino acids 1-43 of Hsap\APP bearing a secretion signal from nec (Tag:SS(nec)).

(Burnouf et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\APP

Tagged with

Tag:SS(nec)

signal sequence

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease

CEA

(Burnouf et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage | RU486 conditional, with Hsap\APP^{Aβ40.UAS.Tag:SS(nec).cBa}, Scer\GAL4^{elav.Switch.PO}

(Burnouf et al., 2015)

abnormal locomotor behavior | adult stage | RU486 conditional, with Hsap\APP^{Aβ42.UAS.Tag:SS(nec).cBa}, Scer\GAL4^{elav.Switch.PO}

(Burnouf et al., 2015)

abnormal locomotor behavior | adult stage | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Burnouf et al., 2015)

short lived | RU486 conditional, with Hsap\APP^{Aβ40.UAS.Tag:SS(nec).cBa}, Scer\GAL4^{elav.Switch.PO}

(Burnouf et al., 2015)

short lived | RU486 conditional, with Hsap\APP^{Aβ42.UAS.Tag:SS(nec).cBa}, Scer\GAL4^{elav.Switch.PO}

(Burnouf et al., 2015)

short lived | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Burnouf et al., 2015)

visible, with Scer\GAL4^GMR.PU

(Burnouf et al., 2015)

visible | adult stage, with Scer\GAL4^GMR.PS

(Yeewa et al., 2024)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PS

(Yeewa et al., 2024)

eye, with Scer\GAL4^GMR.PU

(Burnouf et al., 2015)

photoreceptor neuron | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Burnouf et al., 2015)

Detailed Description

Statement

Reference

Expression of Hsap\APP^{Aβ43.Scer\UAS.T:SS-nec.cBa} under the control of Scer\GAL4^GMR.PU results in ommatidial disorganisation and eye roughening that is less severe than that seen with Hsap\APP^{Aβ42.Scer\UAS.T:SS-nec.cBa}.

Adult-restricted neuronal expression of two copies of Hsap\APP^{Aβ43.Scer\UAS.T:SS-nec.cBa} under the control of Scer\GAL4^{elav.Switch.PO} results in climbing defects and survival that are less severe than those seen with Hsap\APP^{Aβ42.Scer\UAS.T:SS-nec.cBa}. Progressive and pronounced loss of photoreceptor neurons is seen.

Adult-restricted neuronal expression of one copy of Hsap\APP^{Aβ43.Scer\UAS.T:SS-nec.cBa} under the control of Scer\GAL4^{elav.Switch.PO} has no effect on climbing ability or survival.

Adult-restricted neuronal expression of one copy each of Hsap\APP^{Aβ43.Scer\UAS.T:SS-nec.cBa} and Hsap\APP^{Aβ40.Scer\UAS.T:SS-nec.cBa} under the control of Scer\GAL4^{elav.Switch.PO} results in climbing defects and shortened lifespan that are not seen when one copy of either transgene is expressed alone.

Adult-restricted neuronal expression of one copy each of Hsap\APP^{Aβ42.Scer\UAS.T:SS-nec.cBa} and Hsap\APP^{Aβ43.Scer\UAS.T:SS-nec.cBa} under the control of Scer\GAL4^{elav.Switch.PO} results in climbing defects and shortened lifespan that are modestly but significantly less severe than those seen when Hsap\APP^{Aβ42.Scer\UAS.T:SS-nec.cBa} is expressed alone.

(Burnouf et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Hsap\APP^{Aβ43.UAS.Tag:SS(nec).cBa}, Scer\GAL4^GMR.PS has visible | adult stage phenotype, non-enhanceable by Ero1L^HMC05709, Scer\GAL4^GMR.PS

(Yeewa et al., 2024)

NOT suppressed by