In contrast to controls, which adjust their morning and evening activity peaks to align with seasonal dawn and dusk, flies expressing Pura^{dsRNA.shRNA.Scer\UAS} under the control of Scer\GAL4^P0.5.Pdf (restricted to the adult stages using Scer\GAL80^ts.αTub84B) are active later than control flies when exposed to a summer light:dark cycle (14 hours day:10 hours night). Timing of activity is unaffected compared to controls when the flies are exposed to a winter cycle (10 hours day:14 hours night). Normal locomotor activity is seen when the flies are kept in complete darkness (DD conditions), although clock protein rhythms are slightly altered in LNds after three days compared with controls. The rhythms in s-LNvs and DN1s are similar to controls.

Expressing Pura^{dsRNA.shRNA.Scer\UAS} for 12 hours under the control of Scer\GAL4^P0.5.Pdf (restricted to the adult stages using Scer\GAL80^ts.αTub84B) reduces the normally high number of dendrites in LNv projections at ZT14.

Pura^RNAi.UAS, Scer\GAL4^P0.5.Pdf is a suppressor of abnormal circadian behavior phenotype of Rho1^UAS.cMa, Scer\GAL4^P0.5.Pdf