FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\OraiE180A.UAS
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General Information
Symbol
Dmel\OraiE180A.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0316921
Feature type
allele
Associated gene
Dmel\Orai
Associated Insertion(s)
Carried in Construct
P{UAS-Orai.E180A}
Also Known As
dOraiE180A
Key Links
Transgenic product class
missense_variant
(Pathak et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Pathak et al., 2015)
Mutagen
in vitro construct
(Pathak et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-Orai.E180A}
Cytology
Description

UASt regulatory sequences drive expression of a mutant olf186-F cDNA with amino acid substitution E180A.

(Pathak et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Orai
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      When olf186-FE180A.Scer\UAS is expressed under the control of Scer\GAL4ple.PF, store operated calcium entry is significantly reduced in both larval and pupal dopaminergic neurons compared with wild-type. Expression of olf186-FE180A.Scer\UAS does not affect store calcium release in dopaminergic neurons.

      Expression of olf186-FE180A.Scer\UAS in either aminergic neurons (using Scer\GAL4Ddc.PU) or dopaminergic neurons (using Scer\GAL4ple.PF) engenders the complete loss of flight and flight-induced action potentials from dorsal longitudinal muscles. Expression of olf186-FE180A.Scer\UAS in serotonergic neurons (using Scer\GAL4Trh.PU) does not affect flight. General motor function is not impaired by expression of olf186-FE180A.Scer\UAS in dopaminergic neurons, as evident by their ability to climb normally.

      Flies with olf186-FE180A.Scer\UAS expression (using Scer\GAL4ple.TH-C') in THC' dopaminergic neuron sub-domain can fly normally.

      Pupal expression of olf186-FE180A.Scer\UAS in dopaminergic neurons results in complete loss of flight.

      Normal flight bouts are observed upon restricting the expression of olf186-FE180A.Scer\UAS to either larval or adult stages.

      (Pathak et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      OraiE180A.UAS
      dOraiE180A
      (Deb et al., 2016)
      olf186-FE180A.Scer\UAS
      olf186-FE180A.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (3)