Blocking neural transmission in Scer\GAL4⁰⁵⁹⁵ (L3)-expressing neurons results in defects in motion-evoked rotation and translation behavior in population studies in which flies are exposed to random dot motion stimuli.

Blocking neural transmission in Scer\GAL4⁰⁵⁹⁵ (L3)-expressing neurons does not result in defects in turning responses to rotational visual motion.

Flies show no turning at all in response to moving dark edges (a transition from brighter to darker) when both Scer\GAL4^21D (L2) and Scer\GAL4⁰⁵⁹⁵ (L3)-expressing neurons are silenced, and turn more strongly towards light edge motion in an opposing edge assay than when only L2 neurons are silenced. The flies display turning responses to light edges.

In contrast to silencing either neuron alone, when neurons expressing Scer\GAL4^c202a (L1) and Scer\GAL4⁰⁵⁹⁵ (L3) are both silenced the flies show little response to dark edge motion. In addition, these flies are largely unable to respond to rotating square wave gratings containing both edge types.

Blocking neural transmission in both Scer\GAL4⁰⁵⁹⁵ (L3) and Scer\GAL4⁰⁹⁸⁷ (L4)-expressing neurons does not result in defects in turning responses to rotational visual motion.

Blocking neural transmission in Scer\GAL4⁰⁵⁹⁵ (L3)-expressing neurons results in reduced modulation of forward walking speed in response to to translational stimuli compared with controls. Responses to back-to-front moving square gratings are decreased, but responses to front-to-back moving square gratings are indistinguishable from controls.

Flies show very little modulation of forward walking speed in response to front-to-back translational motion when both Scer\GAL4^21D (L2) and Scer\GAL4⁰⁵⁹⁵ (L3)-expressing neurons are silenced and no detectable slowing in response to back-to-front motion.

Additionally silencing Scer\GAL4^c202a (L1)-expressing neurons does not enhance the defects in translational motion response seen when Scer\GAL4⁰⁵⁹⁵ (L3)-expressing neurons are silenced.

Additionally silencing Scer\GAL4⁰⁹⁸⁷ (L4)-expressing neurons does not enhance the defects in translational motion response seen when Scer\GAL4⁰⁵⁹⁵ (L3)-expressing neurons are silenced.