FlyBase Allele Report: Dmel\Lerp[F6]

General Information

Symbol

Dmel\Lerp^F6

Species

D. melanogaster

Name

FlyBase ID

FBal0317017

Feature type

allele

Associated gene

Dmel\Lerp

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Hasanagic et al., 2015)

amorphic allele - molecular evidence

(Hasanagic et al., 2015)

Mutagen

ends-out gene targeting

(Hasanagic et al., 2015)

I-SceI

(Hasanagic et al., 2015)

FLPase

(Hasanagic et al., 2015)

Progenitor genotype

Cytology

Description

Lerp^F6 is the result of a partial internal duplication combined with an insertion that places a nearly intact copy of the donor sequence downstream of the intended target sequence. This results in duplications of the intronic sequences flanking the donor, as well as duplication of 3.5 exons from the 5' cluster of Lerp exons.

(Hasanagic et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Although viable and fertile, both homozygous and hemizygous adult Lerp^F6 mutant flies exhibit a small but statistically significant decrease in body mass relative to wild-type. There is no significant difference in third instar larval weight. Decrease in adult body mass is the only abnormality observed.

Compared with wild-type, homo- and hemizygous Lerp^F6 mutant adult flies display elevated sensitivity to dietary chloroquine exposure. Dietary chloroquine is a drug that raises lysosomal pH and impairs lysosome hydrolytic activity. Although exposure to dietary chloroquine is also lethal to wild-type flies over a period of days, the survival time of mutants upon chloroquine exposure is significantly reduced compared with wild-type.

The median survival time for homo- and hemizygous Lerp^F6 mutant flies during amino acid starvation is reduced compared with wild-type flies under identical conditions.

(Hasanagic et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Lerp^F6 is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, bchs^EP2299

(Hasanagic et al., 2015)

Lerp^F6/Df(3R)BSC524 is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, bchs^EP2299