FlyBase Allele Report: Dmel\cnn[ΔEx1A]

General Information

Symbol

Dmel\cnn^ΔEx1A

Species

D. melanogaster

Name

FlyBase ID

FBal0317020

Feature type

allele

Associated gene

Dmel\cnn

Associated Insertion(s)

Carried in Construct

PBac{cnn^ΔEx1A}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Eisman et al., 2015)

Progenitor genotype

cnn^{+tCH322-189K22}

(Eisman et al., 2015)

Carried in construct

PBac{cnn^ΔEx1A}

Cytology

Description

Recombineering is used to delete the entire exon 1A coding region, the 5'-UTR, and an additional 150 bp upstream of the transcription start site in the cnn BAC. This construct should result in the loss of the three exon 1A transcripts with little or no effect on the downstream gene products expressed from exons 1B and 1C.

(Eisman et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

cnn

Encoded product / tool

cnn

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | maternal effect, with Df(2R)cnn/cnn^HK21

(Eisman et al., 2015)

Phenotype Manifest In

nucleus | embryonic stage | maternal effect, with Df(2R)cnn/cnn^mfs3

(Eisman et al., 2015)

nucleus | embryonic stage | maternal effect, with Df(2R)cnn/cnn^mfs7

(Eisman et al., 2015)

spindle | embryonic stage | maternal effect, with Df(2R)cnn/cnn^mfs3

(Eisman et al., 2015)

spindle | embryonic stage | maternal effect, with Df(2R)cnn/cnn^mfs7

(Eisman et al., 2015)

Detailed Description

Statement

Reference

cnn^ΔEx1A embryos in a cnn^HK21/Df(2R)cnn mutant background fail to cellularize.

Embryos produced by cnn^mfs3/Df(2R)cnn; cnn^ΔEx1A females exhibit multiple nuclei and spindles scattered throughout the embryo (deep and cortical) and frequent nuclear fusions. These embryos produce normal triploid polar bodies following the completion of meiosis. In the mutant embryos, early spindles frequently have an obvious spindle pole, but the poles are lost as development proceeds and barrel-shaped anastral spindles accumulate, accompanied by nuclear fusions.

Embryos produced by cnn^mfs7/Df(2R)cnn; cnn^ΔEx1A females exhibit multiple nuclei and spindles scattered throughout the embryo (deep and cortical) and frequent nuclear fusions. These embryos produce normal triploid polar bodies following the completion of meiosis. In the mutant embryos, early spindles frequently have an obvious spindle pole, but the poles are lost as development proceeds and barrel-shaped anastral spindles accumulate, accompanied by nuclear fusions.

(Eisman et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Dp(2;3)BAC.cnn^+eGFP::Ex1A

(Sanghavi et al., 2016)

cnn^ΔEx1A

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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