FlyBase Allele Report: Dmel\Ilp8[lexAop.cCa]

General Information

Symbol

Dmel\Ilp8^lexAop.cCa

Species

D. melanogaster

Name

Escherichia coli lexAop construct a of Colombani

FlyBase ID

FBal0317082

Feature type

allele

Associated gene

Dmel\Ilp8

Associated Insertion(s)

Carried in Construct

P{lexAop-Ilp8.C}

Key Links

Transgenic product class

cDNA

(Colombani et al., 2015)

wild_type

(Colombani et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Colombani et al., 2015)

wild_type

(Colombani et al., 2015)

Mutagen

in vitro construct

(Colombani et al., 2015)

Progenitor genotype

Carried in construct

P{lexAop-Ilp8.C}

Cytology

Description

lexAop regulatory sequences drive expression of the Ilp8 coding sequence (derived from the IP06470 cDNA).

(Colombani et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

lexAop

binary expression system - regulatory region

Encoded product / tool

Ilp8

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Ecol\lexA^rn-89-lexA

(Colombani et al., 2015)

Phenotype Manifest In

wing disc, with Ecol\lexA^rn-89-lexA

(Colombani et al., 2015)

Detailed Description

Statement

Reference

Expression of Ilp8^{Ecol\lexAop.cCa} under the control of Ecol\lexA^rn-89-lexA in the imaginal tissues results in a developmental delay in pupariation.

Expression of Ilp8^{Ecol\lexAop.cCa} under the control of Ecol\lexA^rn-89-lexA results in a significant decrease in the size of the wing disc compared to controls.

(Colombani et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ecol\lexA^rn-89-lexA, Ilp8^lexAop.cCa has abnormal developmental rate phenotype, suppressible by Scer\GAL4^nSyb.PU/Lgr3^JF03217

(Colombani et al., 2015)

Ecol\lexA^rn-89-lexA, Ilp8^lexAop.cCa has abnormal developmental rate phenotype, suppressible by Scer\GAL4^nSyb.PU/Lgr3^GL01056

(Colombani et al., 2015)

Ecol\lexA^rn-89-lexA, Ilp8^lexAop.cCa has abnormal developmental rate phenotype, suppressible by Lgr3^GL01056/Scer\GAL4^VP16.Lgr3

(Colombani et al., 2015)

Ecol\lexA^rn-89-lexA, Ilp8^lexAop.cCa has abnormal developmental rate phenotype, suppressible by Scer\GAL4^GMR19B09/Lgr3^GL01056

(Colombani et al., 2015)

NOT suppressed by

Statement

Reference

Ecol\lexA^rn-89-lexA, Ilp8^lexAop.cCa has abnormal developmental rate phenotype, non-suppressible by Lgr4^KK106305/Scer\GAL4^nSyb.PU

(Colombani et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ecol\lexA^rn-89-lexA, Ilp8^lexAop.cCa has wing disc phenotype, suppressible by Lgr3^GL01056/Scer\GAL4^VP16.Lgr3

(Colombani et al., 2015)

Ecol\lexA^rn-89-lexA, Ilp8^lexAop.cCa has wing disc phenotype, suppressible by Scer\GAL4^GMR19B09/Lgr3^GL01056

(Colombani et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Lgr3^JF03217 in neurons under the control of Scer\GAL4^nSyb.PU suppresses the developmental delay caused by expression of Ilp8^{Ecol\lexAop.cCa} in the imaginal tissues under the control of Ecol\lexA^rn-89-lexA.

Expression of Lgr3^GL01056 in neurons under the control of either Scer\GAL4^nSyb.PU, Scer\GAL4^{Lgr3.T:Hsim\VP16} or Scer\GAL4^GMR19B09 completely suppresses the developmental delay caused by expression of Ilp8^{Ecol\lexAop.cCa} in the imaginal tissues under the control of Ecol\lexA^rn-89-lexA.

Expression of Lgr4^KK106305 in neurons under the control of Scer\GAL4^nSyb.PU does not suppress the developmental delay caused by expression of Ilp8^{Ecol\lexAop.cCa} in the imaginal tissues under the control of Ecol\lexA^rn-89-lexA.

Expression of Lgr3^GL01056 under the control of either Scer\GAL4^{Lgr3.T:Hsim\VP16} or Scer\GAL4^GMR19B09 completely rescues the reduction in wing disc size caused by expression of Ilp8^{Ecol\lexAop.cCa} under the control of Ecol\lexA^rn-89-lexA.

(Colombani et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ilp8^{Ecol\lexAop.cCa}

Ilp8^lexAop.cCa

Name Synonyms

Escherichia coli lexAop construct a of Colombani

Secondary FlyBase IDs

References (1)

Report Sections

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