FlyBase Allele Report: Dmel\Poxn[BRB]

General Information

Symbol

Dmel\Poxn^BRB

Species

D. melanogaster

Name

FlyBase ID

FBal0317100

Feature type

allele

Associated gene

Dmel\Poxn

Associated Insertion(s)

Mi{Hto}Poxn^BRB

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

minos activity

Nature of the Allele

Allele class

Mutagen

minos activity

(Javeed et al., 2015)

Progenitor genotype

Associated Insertion(s)

Mi{Hto}Poxn^BRB

Cytology

Description

Insertion of a Mi{Hto} element in the last intron of Poxn ( 2R:15 ,829,437 (+) -Release 6). The hostile takeover (hto) exon in the Mi{Hto} element, which contains a T:Zzzz\FLAG tag and the mCherry form of Disc\RFP, splices to the last Poxn exon. This results in a fusion protein being expressed under the control of Scer\UAS regulatory sequences in the Mi{Hto} element.

(Javeed et al., 2015)

Allele components

Component

Use(s)

Inserted element

Mi{Hto}

protein trap

misexpression element

RMCE target element

Regulatory region(s)

UAS

binary expression system - regulatory region

Tagged with

mCherry

red fluorescent protein

Tag:FLAG

epitope tag

Also carries

attP

integrase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:15,829,437..15,829,437 [+]

(Javeed et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color, with Scer\GAL4^GMR.PU

(Javeed et al., 2015)

visible, with Scer\GAL4^Bx-MS1096

(Javeed et al., 2015)

visible, with Scer\GAL4^GMR.PU

(Javeed et al., 2015)

visible, with Scer\GAL4^pnr.PU

(Javeed et al., 2015)

Phenotype Manifest In

abdomen, with Scer\GAL4^pnr.PU

(Javeed et al., 2015)

eye, with Scer\GAL4^GMR.PU

(Javeed et al., 2015)

femur | pupal stage, with Scer\GAL4^ptc-559.1

(Javeed et al., 2015)

macrochaeta | increased number, with Scer\GAL4^pnr.PU

(Javeed et al., 2015)

thorax, with Scer\GAL4^pnr.PU

(Javeed et al., 2015)

tibia | pupal stage, with Scer\GAL4^ptc-559.1

(Javeed et al., 2015)

wing vein | ectopic, with Scer\GAL4^Bx-MS1096

(Javeed et al., 2015)

Detailed Description

Statement

Reference

Expression of one copy of Poxn^BRB under the control of Scer\GAL4^GMR.PU produces flies that have pale/mottled and glazed (lacking clear separation of ommatidia) eyes.

Expression of Poxn^BRB under the control of Scer\GAL4^Bx-MS1096 results in the production of extra vein material, especially between L3 and L4 and near L5.

Expression of Poxn^BRB under the control of Scer\GAL4^pnr.PU severely impairs thorax development and eliminates abdominal pigment bands. Although the thorax does not expand properly, the number of macrochaetae in the Scer\GAL4^pnr.PU-expressing zone of the thorax roughly doubles.

Expression of Poxn^BRB under the control of Scer\GAL4^ptc-559.1 results in failure of the femur and tibia to elongate in pupal stage flies.

(Javeed et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO has visible phenotype, non-enhanceable by ct^JF03304, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO has abnormal eye color phenotype, non-enhanceable by ct^JF03304, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Poxn^BRB, Scer\GAL4^GMR.PU has visible phenotype, non-enhanceable by ct^BRO, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Poxn^BRB, Scer\GAL4^GMR.PU has abnormal eye color phenotype, non-enhanceable by ct^BRO, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

NOT suppressed by

Statement

Reference

Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO has visible phenotype, non-suppressible by ct^JF03304, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO has abnormal eye color phenotype, non-suppressible by ct^JF03304, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Poxn^BRB, Scer\GAL4^Bx-MS1096 has visible phenotype, non-suppressible by ct^JF03304, Scer\GAL4^Bx-MS1096

(Javeed et al., 2015)

NOT Enhancer of

Statement

Reference

Poxn^BRB, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PU, ct^JF03304

(Javeed et al., 2015)

Poxn^BRB, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PU, ct^BRO

(Javeed et al., 2015)

Poxn^BRB, Scer\GAL4^GMR.PU is a non-enhancer of abnormal eye color phenotype of Scer\GAL4^GMR.PU, ct^BRO

(Javeed et al., 2015)

NOT Suppressor of

Statement

Reference

Poxn^BRB, Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PU, ct^JF03304

(Javeed et al., 2015)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO has eye phenotype, non-enhanceable by ct^JF03304, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Poxn^BRB, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by ct^BRO, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

NOT suppressed by

Statement

Reference

Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO has eye phenotype, non-suppressible by ct^JF03304, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Poxn^BRB, Scer\GAL4^Bx-MS1096 has wing vein | ectopic phenotype, non-suppressible by ct^JF03304, Scer\GAL4^Bx-MS1096

(Javeed et al., 2015)

NOT Enhancer of

Statement

Reference

Poxn^BRB, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PU, ct^BRO

(Javeed et al., 2015)

Poxn^BRB, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PU, ct^JF03304

(Javeed et al., 2015)

NOT Suppressor of

Statement

Reference

Poxn^BRB, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, ct^JF03304

(Javeed et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Poxn^BRB and ct^BRO under the control of Scer\GAL4^GMR.PU produces an eye phenotype that is qualitatively very similar to when either transgene is expressed alone. This phenotype is not suppressed upon co-expression of ct^JF03304.

Expression of ct^JF03304 does not suppress the wing phenotypes seen when Poxn^BRB is expressed under the control of Scer\GAL4^Bx-MS1096.

(Javeed et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Poxn^BRB is partially rescued by Scer\GAL4^GMR.PU/Poxn^JF02136

(Javeed et al., 2015)

Comments

Expression of Poxn^JF02136 almost completely rescues the eye phenotypes seen when Poxn^BRB is expressed under the control of Scer\GAL4^GMR.PU, although the posterior region remains mispatterned.

(Javeed et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Generated using the hostile takeover (Hto) system.

(Javeed et al., 2015)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Poxn^BRB

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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