FlyBase Allele Report: Dmel\ct[BRO]

General Information

Symbol

Dmel\ct^BRO

Species

D. melanogaster

Name

FlyBase ID

FBal0295094

Feature type

allele

Associated gene

Dmel\ct

Associated Insertion(s)

Mi{Hto-WP}ct^BRO

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

minos activity

Nature of the Allele

Allele class

Mutagen

minos activity

(Singari et al., 2014)

Progenitor genotype

Associated Insertion(s)

Mi{Hto-WP}ct^BRO

Cytology

Description

Insertion of a Mi{Hto-WP} element upstream of ct ( X:7 ,581,688 (+) -Release 6). The hostile takeover (hto) exon in the Mi{Hto-WP} element splices to the downstream ct exon 3. This results in a fusion protein being expressed under the control of Scer\UAS regulatory sequences in the Mi{Hto-WP} element. The fusion protein consists of 3 copies of the T:Zzzz\FLAG tag, the mCherry form of Disc\RFP and the coding sequence of the ct-RC isoform.

(Javeed et al., 2015)

Allele components

Component

Use(s)

Inserted element

Mi{Hto-WP}

protein trap

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Tagged with

mCherry

red fluorescent protein

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:7,581,688..7,581,688 [+]

(Özel et al., 2022, Javeed et al., 2015, Singari et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color, with Scer\GAL4^GMR.PU

(Javeed et al., 2015)

lethal - all die before end of pupal stage, with Scer\GAL4^ptc.PU

(Javeed et al., 2015)

majority die during P-stage, with Scer\GAL4^ey.PU

(Javeed et al., 2015)

partially lethal - majority die, with Scer\GAL4^ey.PU

(Javeed et al., 2015)

visible, with Scer\GAL4^Bx-MS1096

(Javeed et al., 2015)

visible, with Scer\GAL4^Bx-MS1096-KE

(Singari et al., 2014)

visible, with Scer\GAL4^ey.PH

(Singari et al., 2014)

visible, with Scer\GAL4^GMR.PF

(Singari et al., 2014)

visible, with Scer\GAL4^GMR.PU

(Javeed et al., 2015)

visible | pharate adult stage, with Scer\GAL4^ey.PU

(Javeed et al., 2015)

Phenotype Manifest In

alar lobe, with Scer\GAL4^Bx-MS1096

(Javeed et al., 2015)

antenna | ectopic | pharate adult stage, with Scer\GAL4^ey.PU

(Javeed et al., 2015)

eye, with Scer\GAL4^ey.PH

(Singari et al., 2014)

eye, with Scer\GAL4^GMR.PF

(Singari et al., 2014)

eye, with Scer\GAL4^GMR.PU

(Javeed et al., 2015)

eye | pharate adult stage, with Scer\GAL4^ey.PU

(Javeed et al., 2015)

leg | pupal stage, with Scer\GAL4^ptc.PU

(Javeed et al., 2015)

wing, with Scer\GAL4^Bx-MS1096

(Javeed et al., 2015)

wing, with Scer\GAL4^Bx-MS1096-KE

(Singari et al., 2014)

Detailed Description

Statement

Reference

Expression of one copy of ct^BRO under the control of Scer\GAL4^GMR.PU produces flies that have pale/mottled and glazed (lacking clear separation of ommatidia) eyes.

Most flies expressing ct^BRO under the control of Scer\GAL4^ey.PU die during the pharate adult stage. Most of the pharate adult flies examined exhibit partial to full conversion of eye to antenna.

When ct^BRO is expressed in the wing blade region under the control of Scer\GAL4^Bx-MS1096 the distal part of the wing margin is strongly contracted and in the proximal part of the wing the alula is very reduced.

The legs of pupal stage flies expressing ct^BRO under the control of Scer\GAL4^ptc.PU show severe morphological changes.

(Javeed et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO has visible phenotype, non-enhanceable by ct^JF03304, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO has abnormal eye color phenotype, non-enhanceable by ct^JF03304, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Scer\GAL4^GMR.PU, ct^BRO has visible phenotype, non-enhanceable by Poxn^BRB, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Scer\GAL4^GMR.PU, ct^BRO has abnormal eye color phenotype, non-enhanceable by Poxn^BRB, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

NOT suppressed by

Statement

Reference

Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO has visible phenotype, non-suppressible by ct^JF03304, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO has abnormal eye color phenotype, non-suppressible by ct^JF03304, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

NOT Enhancer of

Statement

Reference

ct^BRO, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Poxn^BRB, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

ct^BRO, Scer\GAL4^GMR.PU is a non-enhancer of abnormal eye color phenotype of Poxn^BRB, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO has eye phenotype, non-enhanceable by ct^JF03304, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Scer\GAL4^GMR.PU, ct^BRO has eye phenotype, non-enhanceable by Poxn^BRB, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

NOT suppressed by

Statement

Reference

Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO has eye phenotype, non-suppressible by ct^JF03304, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

NOT Enhancer of

Statement

Reference

ct^BRO, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Poxn^BRB, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Poxn^BRB and ct^BRO under the control of Scer\GAL4^GMR.PU produces an eye phenotype that is qualitatively very similar to when either transgene is expressed alone. This phenotype is not suppressed upon co-expression of ct^JF03304.

(Javeed et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

ct^BRO is partially rescued by ct^JF03304/Scer\GAL4^GMR.PU

(Javeed et al., 2015)

ct^BRO is partially rescued by ct^JF03304/Scer\GAL4^Bx-MS1096

(Javeed et al., 2015)

Rescues

ct^BRO/Scer\GAL4^GMR.PU rescues ct^JF03304

(Javeed et al., 2015)

Comments

Expression of ct^JF03304 substantially rescues the wing phenotypes seen when ct^BRO is expressed under the control of Scer\GAL4^Bx-MS1096. Only minor defects remain, such as a weak posterior margin.

Co-expression of ct^BRO and ct^JF03304 under the control of Scer\GAL4^GMR.PU suppresses the eye phenotypes seen when either transgene is expressed alone. Only a slight reduction in pigmentation remains.

(Javeed et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

56538

w^* Mi{Hto-WP}ct^BRO

Notes on Origin

Discoverer

Comments

Generated using the hostile takeover (Hto) system.

(Javeed et al., 2015)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ct^BRO

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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