FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\ouib74
Open Close
General Information
Symbol
Dmel\ouib74
Species
D. melanogaster
Name
FlyBase ID
FBal0318067
Feature type
allele
Associated gene
Dmel\ouib
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Komura-Kawa et al., 2015)
Progenitor genotype
Cytology
Description

A 7bp deletion that results in a premature stop codon in the putative coding sequence of ouib. The predicted protein is 74 amino acids long.

(Komura-Kawa et al., 2015)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3R:8,725,670..8,725,676 [+]
Comment:

7 bp deletion in the ouib coding sequence that results in a frameshift and premature translation termination.

(Komura-Kawa et al., 2015)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      ouib29/ouib74 mutant embryos complete embryogenesis, hatch normally and show no apparent morphological defects after hatching. They arrest development in the first instar larval stage and never molt into second instars. All ouib29/ouib74 mutant larvae die by 144 hours after egg laying. The majority of heterozygous animals become pupae.

      The ecdysteroid titer in ouib29/ouib74 mutant animals is significantly reduced. When fed yeast paste containing 20E after hatching they are able to molt to the second instar larval stage or later.

      When ouib29/ouib74 mutants are fed yeast paste supplemented with cholesterol or 7-dehydrocholesterol (7dC), the larvae still arrest at the first instar larval stage. The larval arrest phenotype is rescued when the animals are fed yeast paste supplemented with 5β-ketodiol (5βkd).

      ouib74/Df(3R)ED5330 mutant animals arrest at the first instar larval stage but are able to reach the second instar stage when fed yeast paste containing 20-hydroxyecdysone (20E).

      (Komura-Kawa et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of spoScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4phm.PU suppresses the first instar larval arrest phenotype seen in ouib29/ouib74 mutants.

      (Komura-Kawa et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      ouib74
      (Komura-Kawa et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)