FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\obe2
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General Information
Symbol
Dmel\obe2
Species
D. melanogaster
Name
FlyBase ID
FBal0318086
Feature type
allele
Associated gene
Dmel\obe
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Vichas et al., 2015)
Mutagen
spontaneous
(Vichas et al., 2015)
Progenitor genotype
Cytology
Description

19bp deletion in the second obe exon and a C173T point mutation. These changes introduce a frameshift after amino acid 48 and a stop codon at amino acid 52.

(Vichas et al., 2015)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3R:15,486,345..15,486,363 [+]
Comment:

19 base deletion in the second exon of obe which causes a frameshift. One of two lesions in mutant.

(Vichas et al., 2015)
point_mutation
3R:15,486,369..15,486,369 [+]
Nucleotide change:

C15486369T

Reported nucleotide change:

C173T

Amino acid change:

P58L | obe-PA

Comment:

A single base change causing a stop codon in the mutant (in the context of the 19 bp frameshift-inducing deletion just upstream also found in the @obe[2] mutant). There is also a C to T polymorphism between the reported sequence and the reference genome sequence 8 bases upstream of this point mutation.

(Vichas et al., 2015)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos derived from obe1/obe2 females show significantly reduced axis elongation during germband extension compared to wild type (the severity of the defect is unaffected by the paternal obe contribution).

      obe2/Df(3R)Exel6174 embryos derived from obe2/Df(3R)Exel6174 females crossed to obe2/+ males show significantly reduced axis elongation during germband extension compared to wild type.

      obe2/Df(3R)Exel6174 embryos derived from obe2/Df(3R)Exel6174 females crossed to obe2/+ males and obe1/obe2 embryos derived from obe1/obe2 females crossed to obe2/+ males show an abnormal aggregation of adherens junction proteins at stage 7.

      In contrast to wild type, most of the centrosomes in epithelial cells fail to dissociate from microtubules and remain in close proximity to adherens junctions throughout axis elongation (stages 6 and 7) in obe1/obe2 embryos derived from obe1/obe2 females crossed to obe2/+ males. This results in a failure of the centrosomes to relocalise laterally. The mis-positioned centrosomes are often associated with adherens junction aggregates.

      The adherens junction and centrosome defects seen in stage 7 obe1/obe2 embryos derived from obe1/obe2 females crossed to obe2/+ males are rescued by nocodazole treatment (which inhibits microtubule polymerisation).

      (Vichas et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      obe1/obe2 is rescued by obeCH322-15H13

      (Vichas et al., 2015)

      obe1/obe2 is rescued by obesqh.PV

      (Vichas et al., 2015)
      Comments

      Expression of obeCH322-15H13 rescues the axis elongation defects seen during germband extension in the progeny of obe1/obe1 and obe1/obe2 females crossed to obe1/+ males.

      (Vichas et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      Separable mutation isolated in the P{XP}Surf4d04274 insertion line.

      (Vichas et al., 2015)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      obe2
      (Vichas et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)