UASt regulatory sequences drive expression of spn-F lacking the CC3 coiled-coil domain. The protein is tagged with EGFP.
Scer\GAL4ppk.PG/spn-FΔCC3.UAS.EGFP fails to rescue spn-F2
Expression of spn-FΔCC3.Scer\UAS.T:Avic\GFP-EGFP driven by Scer\GAL4ppk.PG fails to rescue dendrite pruning defects in spn-F2/spn-F2 pupae (16h after puparium formation).