FB2026_02 , released June 18, 2026
Allele: Dmel\Mcm10SJ
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General Information
Symbol
Dmel\Mcm10SJ
Species
D. melanogaster
Name
FlyBase ID
FBal0318486
Feature type
allele
Associated gene
Dmel\Mcm10
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

hypomorphic allele - molecular evidence

Mutagen
    Nature of the Allele
    Allele class

    hypomorphic allele - molecular evidence

    (Reubens et al., 2015)
    Mutagen
    Progenitor genotype
    Cytology
    Description

    G to A nucleotide substitution at the first base of the intron (this corresponds to 1162bp downstream of the ATG start codon). This change is thought to result in read-through into the intron until a stop codon is encountered 1191bp downstream of the ATG start codon, generating a 389 amino acid residue protein. (Revision of data reported in FBrf0230407).

    (Reubens, 2016.5.19)

    Transcript levels are reduced compared to wild type; no band representing either a wild type or truncated translated product is detected on a Western blot.

    (Reubens et al., 2015)
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    point_mutation
    2L:21,181,193..21,181,193 [+]
    Nucleotide change:

    G21181193A

    Comment:

    G to A nucleotide change in the first base of the Mcm10 intron appears to result in read-through of the intron and early translation termination 1192 bp downstream of the ATG.

    (Reubens, 2016.5.19, Reubens et al., 2015)
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        Mcm10SJ/Df(2L)Exel6047 are viable and do not show increased adult mortality rates compared to heterozygote controls. Mcm10SJ/Df(2L)Exel6047 have reduced female fecundity.

        Percentage of Mcm10SJ/Df(2L)Exel6047 embryos hatching is increased with wild type maternal (and slightly by paternal) allele donation.

        Mcm10SJ/Df(2L)Exel6047 are seen in early embryonic cell cycle stages significantly more than wild type, mutant pre-gastrulating embryos have chromosome fragmentation, females show reductions in egg deposition compared to wild type and frequently (with incomplete penetrance and sometimes asymmetric) display small ovaries largely devoid of vitellogenic follicles. In contrast to wild type ovarioles, Mcm10SJ/Df(2L)Exel6047 display long thin strings of somatic cells.

        (Reubens et al., 2015)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Phenotype Manifest In
        Additional Comments
        Genetic Interactions
        Statement
        Reference
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Partially rescued by

        Df(2L)Exel6047/Mcm10SJ is partially rescued by Scer\GAL4VP16.nanos.UTR/Mcm10UAS.cRa

        (Reubens et al., 2015)
        Comments

        Mcm10Scer\UAS.cRa driven by Scer\GAL4nos.UTR.T:Hsim\VP16 partially rescues reduced fecundity, percentage of embryos hatching and early embryonic cell cycle deficiencies in Mcm10SJ/Df(2L)Exel6047 female flies.

        (Reubens et al., 2015)
        Images (0)
        Mutant
        Wild-type
        Stocks (0)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (1)
        Reported As
        Symbol Synonym
        Mcm10SJ
        (Reubens, 2016.5.19, Reubens et al., 2015)
        Name Synonyms
        Secondary FlyBase IDs
          References (2)