FlyBase Allele Report: Dmel\fs(1)h[UAS.EGFP]

General Information

Symbol

Dmel\fs(1)h^UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0318512

Feature type

allele

Associated gene

Dmel\fs(1)h

Associated Insertion(s)

Carried in Construct

PBac{UAS-fs(1)h.S.EGFP}

Key Links

Transgenic product class

cDNA

(Bagley et al., 2014)

wild_type

(Bagley et al., 2014)

Mutagen

Nature of the Allele

Transgenic product class

cDNA

(Bagley et al., 2014)

wild_type

(Bagley et al., 2014)

Mutagen

Progenitor genotype

Carried in construct

PBac{UAS-fs(1)h.S.EGFP}

Cytology

Description

UAS regulatory sequences drive expression of the fs(1)h S isoform (derived from cDNA clone LD26482) that is tagged at the C-terminal end with EGFP.

(Bagley et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

fs(1)h

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^109(2)80

(Bagley et al., 2014)

Phenotype Manifest In

dendrite | third instar larval stage, with Scer\GAL4^109(2)80

(Bagley et al., 2014)

larval multidendritic class III neuron | third instar larval stage, with Scer\GAL4^109(2)80

(Bagley et al., 2014)

larval multidendritic class IV neuron | third instar larval stage, with Scer\GAL4^109(2)80

(Bagley et al., 2014)

Detailed Description

Statement

Reference

fs(1)h^{Scer\UAS.T:Avic\GFP-EGFP} overexpression driven by Scer\GAL4^109(2)80 (at 25[o]C or 20[o]C) causes significant reduction of dendritic spikes (but no change in primary dendrite branch length or branch points) in larval class III da sensory neurons.

fs(1)h^{Scer\UAS.T:Avic\GFP-EGFP} overexpression driven by Scer\GAL4^109(2)80 or Scer\GAL4^2-21 does not affect dendrite length or branch points in larval class I da sensory neurons.

fs(1)h^{Scer\UAS.T:Avic\GFP-EGFP} overexpression driven by Scer\GAL4^109(2)80 significantly decreases dendrite branch length and branch points in in larval class IV da sensory neurons.

(Bagley et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

fs(1)h^UAS.EGFP/Scer\GAL4²²¹ is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4²²¹, ct^UAS.cUa

(Bagley et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

fs(1)h^UAS.EGFP/Scer\GAL4²²¹ is a suppressor | partially of dendrite | third instar larval stage phenotype of Scer\GAL4^109(2)80, ct^UAS.cUa

(Bagley et al., 2014)

fs(1)h^UAS.EGFP/Scer\GAL4²²¹ is a suppressor | partially of larval multidendritic class I neuron | third instar larval stage phenotype of Scer\GAL4^109(2)80, ct^UAS.cUa

(Bagley et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^109(2)80/fs(1)h^UAS.EGFP partially rescues fs(1)h¹¹¹²

(Bagley et al., 2014)

Comments

Expression of fs(1)h^{Scer\UAS.T:Avic\GFP-EGFP} driven by Scer\GAL4^109(2)80 partially rescues (at 25[o]C or 20[o]C) the reduction of dendritic spikes (but not the reduction in primary dendrite branch length and branch points) in class III da sensory neuron fs(1)h¹¹¹²/fs(1)h¹¹¹² larval clones.

Expression of fs(1)h^{Scer\UAS.T:Avic\GFP-EGFP} driven by Scer\GAL4^109(2)80 partially rescues reduced class IV dendrite length and number, and fails to rescue class II reduced branch points or class I reduced dendrite length, seen in fs(1)h¹¹¹²/fs(1)h¹¹¹² larval clones.

(Bagley et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

fs(1)h^{Scer\UAS.T:Avic\GFP-EGFP}

fs(1)h^UAS.EGFP

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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