FlyBase Allele Report: Dmel\Whamy[Δ109]

General Information

Symbol

Dmel\Whamy^Δ109

Species

D. melanogaster

Name

FlyBase ID

FBal0318551

Feature type

allele

Associated gene

Dmel\Whamy

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Brinkmann et al., 2016)

Mutagen

P-element activity

(Brinkmann et al., 2016)

Progenitor genotype

Cytology

Description

Deletion that removes part of Whamy (exons 3 and 4 are completely deleted, part of exon 2 is deleted).

(Brinkmann et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

lateral transverse muscle cell | late embryonic stage

(Brinkmann et al., 2016)

Detailed Description

Statement

Reference

Isolated pupal macrophages (plated on cover slips) deficient of Whamy^Δ109/Whamy^Δ109 show defects in cell flattening and spreading, resulting in significantly smaller hemocytes compared to wild type.

Whamy^Δ109/Whamy^Δ109 prepupal (2h after puparium formation - APF) macrophage size is significantly decreased and macrophage number is significantly increased compared to wild type. Whamy^Δ109/Whamy^Δ109 prepupal macrophage migration speed is significantly decreased compared to wild type, and mutant macrophages remain closely clustered together; prepupal mutant macrophages show directional migration towards wounds, though at a significantly reduced speed.

Whamy^Δ109/Whamy^Δ109 pupal (17h APF) macrophages respond and migrate to wound sites but their speed is reduced compared to wild type.

Whamy^Δ109/Whamy^Δ109 late stage embryos show attachment defects of lateral transverse muscles.

(Brinkmann et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Whamy^Δ109/Whamy[+] is an enhancer of increased cell number | pupal stage phenotype of Df(3R)3450/WASp¹

(Brinkmann et al., 2016)

Whamy^Δ109/Whamy[+] is an enhancer of abnormal neuroanatomy | pupal stage phenotype of Df(3R)3450/WASp¹

(Brinkmann et al., 2016)

Other

Statement

Reference

WASp¹, Whamy^Δ109 has partially lethal phenotype

(Brinkmann et al., 2016)

Df(3R)3450/WASp¹, Whamy^Δ109/Whamy[+] has partially lethal phenotype

(Brinkmann et al., 2016)

Phenotype Manifest In

Enhancer of

Statement

Reference

Whamy^Δ109/Whamy[+] is an enhancer of macrochaeta | immature adult stage phenotype of Df(3R)3450/WASp¹

(Brinkmann et al., 2016)

Whamy^Δ109/Whamy[+] is an enhancer of external sensory organ precursor cell | pupal stage phenotype of Df(3R)3450/WASp¹

(Brinkmann et al., 2016)

Other

Statement

Reference

WASp¹, Whamy^Δ109 has fusion competent cell | embryonic stage phenotype

(Brinkmann et al., 2016)

WASp¹, Whamy^Δ109 has larval somatic muscle cell | late embryonic stage phenotype

(Brinkmann et al., 2016)

WASp¹, Whamy^Δ109 has segment border muscle cell | late embryonic stage phenotype

(Brinkmann et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Presence of Whamy^Δ109/Whamy^Δ109 enhances early adulthood lethality seen in in WASp¹/WASp¹, whereby double mutants are late-embryonic lethal with few larval escapers.

Whamy^Δ109/+ shifts lethality from late pupal or adult to early larval stages in WASp¹/Df(3R)3450 transheterozygotes. Whamy^Δ109/+ enhances the decrease of macrochaetes on the head and notum of WASp¹/Df(3R)3450 immature adults and the increased number of neurons per sensory organ in WASp¹/Df(3R)3450 (24h APF) pupal notae.

WASp¹/WASp¹;Whamy^Δ109/Whamy^Δ109 double mutant stage 16 embryos have strong defects in muscle size and morphology, including a reduction in the number of nuclei contained in segmental border muscle (disturbed myoblast fusion). WASp¹/WASp¹;Whamy^Δ109/Whamy^Δ109 double mutant stage 11 embryos have similar numbers of founder cells compared to wild type.

(Brinkmann et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Whamy^Δ109 is rescued by Whamy^UAS.EGFP/Scer\GAL4^unspecified

(Brinkmann et al., 2016)

Comments

Expression of Whamy^{Scer\UAS.T:Avic\GFP-EGFP} rescues cell size defects in Whamy^Δ109/Whamy^Δ109 isolated pupal macrophages (plated on a cover slip).

(Brinkmann et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Whamy^Δ109

whamy^Δ109

(Brinkmann et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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