FlyBase Allele Report: Dmel\ics[UAS.cOa]

General Information

Symbol

Dmel\ics^UAS.cOa

Species

D. melanogaster

Name

FlyBase ID

FBal0318752

Feature type

allele

Associated gene

Dmel\ics

Associated Insertion(s)

Carried in Construct

P{UAS-ics.O}

Key Links

Transgenic product class

cDNA

(Ojelade et al., 2015)

wild_type

(Ojelade et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Ojelade et al., 2015)

wild_type

(Ojelade et al., 2015)

Mutagen

in vitro construct

(Ojelade et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-ics.O}

Cytology

Description

UAS regulatory sequences drive expression of ics (sequence derived from LD43981 cDNA clone).

(Ojelade et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

ics

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^ics-G4/ics^UAS.cOa rescues ics^G4/Df(2L)BSC147

(Ojelade et al., 2015)

Scer\GAL4^ics-G4/ics^UAS.cOa rescues ics^G4

(Ojelade et al., 2015)

Scer\GAL4^elav.PU/ics^UAS.cOa rescues ics^x5

(Ojelade et al., 2015)

Partially rescues

Scer\GAL80^Mef2.mb247, Scer\GAL4^ics-G4, ics^UAS.cOa partially rescues ics^G4

(Ojelade et al., 2015)

Fails to rescue

Scer\GAL4^ics-G4, Scer\GAL80^elav.PY, ics^UAS.cOa fails to rescue ics^G4

(Ojelade et al., 2015)

Comments

Expression of ics^Scer\UAS.cOa driven by Scer\GAL4^ics-G4 rescues resistance to ethanol-induced sedation in ics^G4/ics^G4 (or ics^G4/Df(2L)BSC147) flies to control levels; there is no rescue when Scer\GAL80^elav.PY (to suppress neuronal ics expression) is also present.

Expression of ics^Scer\UAS.cOa driven by Scer\GAL4^elav.PU rescues resistance to ethanol-induced sedation in ics^x5/ics^x5 flies to wild type levels.

Expression of ics^Scer\UAS.cOa driven by Scer\GAL4^ics-G4 (using Scer\GAL80^ts.αTub84B to control timing of expression) during adulthood alone (but not throughout development alone) rescues resistance to ethanol-induced sedation in ics^G4/ics^G4 flies to control levels.

Expression of ics^Scer\UAS.cOa driven by Scer\GAL4^ics-G4 restores ethanol consumption behavior in ics^G4/ics^G4 flies to wild type levels; flies with additional presence of Scer\GAL80^Mef2.mb247 (to suppress ics expression in the mushroom bodies) show normal naive ethanol preference and normal resistance to ethanol-induced sedation, but unlike wild type do not acquire a preference for ethanol over 4 days.

(Ojelade et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

66566

w^*; P{UAS-ics.O}3

66567

w^*; P{UAS-ics.O}2

Notes on Origin

Discoverer