A GFP tag is introduced at the ATG of the mud open reading frame in the CH322-147E14 BAC genomic clone. This BAC clone contains ~19.5 kb of X chromosome genomic region including ~3.3 kb upstream of the mud mRNA 5' and 5.4 kb downstream of the mud 3' mRNA and thus most of the coding regions of genes proximal and distal to mud locus. The region encoding the pins binding domain (amino acids 1,928-1,982) of mud is deleted.
mudΔPINS.GFP rescues mud3/Df(1)BSC705
mudΔPINS.GFP rescues Df(1)BSC705/mud4
mudΔPINS.GFP partially rescues mud4