UASt regulatory sequences drive expression of the full-length Gli AE27.41 cDNA. Two copies of the Tag:HA tag have been inserted just before the PDZ-binding motif.
Expression of GliScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4da.PU fully rescues the embryonic lethality of GliAE2Δ45 homozygotes. 50% of the rescued adults have leg defects.