UASt regulatory sequences drive expression of Gli which lacks the PDZ-binding domain. The protein is tagged at the C-terminal end with two copies of Tag:HA.
Scer\GAL4da.PU/GliΔPDZ.UAS.Tag:HA partially rescues GliAE2Δ45
Expression of GliΔPDZ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4da.PU fully rescues the embryonic lethality of GliAE2Δ45 homozygotes. 100% of the rescued adults have leg defects.