FlyBase Allele Report: Dmel\SCCRO4[UAS.Tag:MYC]

General Information

Symbol

Dmel\SCCRO4^UAS.Tag:MYC

Species

D. melanogaster

Name

FlyBase ID

FBal0319337

Feature type

allele

Associated gene

Dmel\SCCRO4

Associated Insertion(s)

Carried in Construct

P{UAS-SCCRO4.Myc}

Key Links

Transgenic product class

wild_type

(Fu et al., 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Fu et al., 2016)

Mutagen

in vitro construct

(Fu et al., 2016)

Progenitor genotype

Carried in construct

P{UAS-SCCRO4.Myc}

Cytology

Description

UASt regulatory sequences drive expression of full-length SCCRO4 which is tagged at the N-terminal end with Tag:MYC.

(Fu et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

SCCRO4

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage, with Scer\GAL4^ptc.PU

(Fu et al., 2016)

decreased cell size | adult stage, with Scer\GAL4^ptc.PU

(Fu et al., 2016)

increased cell death | somatic clone | third instar larval stage

(Fu et al., 2016)

visible, with Scer\GAL4^GMR.PF

(Fu et al., 2016)

Phenotype Manifest In

anterior-posterior compartment boundary of the wing blade, with Scer\GAL4^ptc.PU

(Fu et al., 2016)

eye, with Scer\GAL4^GMR.PF

(Fu et al., 2016)

eye disc | somatic clone | third instar larval stage

(Fu et al., 2016)

macrochaeta, with Scer\GAL4^ptc.PU

(Fu et al., 2016)

Detailed Description

Statement

Reference

Over-expression of one copy of SCCRO4^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^GMR.PF results in smaller, rough eyes at room temperature (over-expression of four copies leads to smaller eyes with severe roughness and dark pigmentation on the surface of some ommatidia). Over-expression of SCCRO4^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^ptc.PU significantly decreases cell number and cell size and significantly reduces the distance between wing veins L3 and L4, compared to controls. Over-expression of SCCRO4^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^ptc.PU leads to missing and smaller macrochaetae on the notum. Clones from third instar larval eye discs with SCCRO4^{Scer\UAS.T:Hsap\MYC} over-expression show increases in apoptosis.

(Fu et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

SCCRO4^UAS.Tag:MYC, Scer\GAL4^ptc.PU has decreased cell number | adult stage phenotype, suppressible by SCCRO3^UAS.Tag:MYC/Scer\GAL4^GMR.PF

(Fu et al., 2016)

SCCRO4^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, suppressible by SCCRO^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Fu et al., 2016)

SCCRO4^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, suppressible | partially by SCCRO3^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Fu et al., 2016)

SCCRO4^UAS.Tag:MYC, Scer\GAL4^ptc.PU has decreased cell number | adult stage phenotype, suppressible by SCCRO^UAS.Tag:HA/Scer\GAL4^GMR.PF

(Fu et al., 2016)

SCCRO4^UAS.Tag:MYC, Scer\GAL4^ptc.PU has decreased cell size | adult stage phenotype, suppressible by SCCRO^UAS.Tag:HA/Scer\GAL4^GMR.PF

(Fu et al., 2016)

NOT suppressed by

Statement

Reference

SCCRO4^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by SCCRO^DAD.UAS, Scer\GAL4^GMR.PF

(Fu et al., 2016)

SCCRO4^UAS.Tag:MYC, Scer\GAL4^ptc.PU has decreased cell number | adult stage phenotype, non-suppressible by SCCRO^DAD.UAS/Scer\GAL4^GMR.PF

(Fu et al., 2016)

SCCRO4^UAS.Tag:MYC, Scer\GAL4^ptc.PU has decreased cell size | adult stage phenotype, non-suppressible by SCCRO^DAD.UAS/Scer\GAL4^GMR.PF

(Fu et al., 2016)

SCCRO4^UAS.Tag:MYC, Scer\GAL4^ptc.PU has decreased cell size | adult stage phenotype, non-suppressible by SCCRO3^UAS.Tag:MYC/Scer\GAL4^GMR.PF

(Fu et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

SCCRO4^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, suppressible by SCCRO^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Fu et al., 2016)

SCCRO4^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by SCCRO3^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Fu et al., 2016)

SCCRO4^UAS.Tag:MYC, Scer\GAL4^ptc.PU has anterior-posterior compartment boundary of the wing blade phenotype, suppressible by SCCRO^UAS.Tag:HA/Scer\GAL4^GMR.PF

(Fu et al., 2016)

SCCRO4^UAS.Tag:MYC, Scer\GAL4^ptc.PU has anterior-posterior compartment boundary of the wing blade phenotype, suppressible | partially by SCCRO3^UAS.Tag:MYC/Scer\GAL4^GMR.PF

(Fu et al., 2016)

NOT suppressed by

Statement

Reference

SCCRO4^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by SCCRO^DAD.UAS, Scer\GAL4^GMR.PF

(Fu et al., 2016)

SCCRO4^UAS.Tag:MYC, Scer\GAL4^ptc.PU has anterior-posterior compartment boundary of the wing blade phenotype, non-suppressible by SCCRO^DAD.UAS/Scer\GAL4^GMR.PF

(Fu et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of SCCRO^{Scer\UAS.T:Ivir\HA1} but not SCCRO^DAD.Scer\UAS suppresses the small rough eye phenotype and wing phenotypes seen in flies with expression of SCCRO4^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^GMR.PF. Co-expression of SCCRO3^{Scer\UAS.T:Hsap\MYC} partially suppresses the small rough eye phenotype seen in flies with expression of SCCRO4^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^GMR.PF. Co-expression of SCCRO3^{Scer\UAS.T:Hsap\MYC} partially suppresses the reduction in distance between L3 and L4 in the wing blade (but does not suppress the decreases in cell size, and suppresses the decreases in cell number even increasing them above control levels) of flies with expression of SCCRO4^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^ptc.PU.

(Fu et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

SCCRO4^{Scer\UAS.T:Hsap\MYC}

SCCRO4^UAS.Tag:MYC

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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