A 500bp fragment containing the 5'UTR and upstream promoter region of the testis isoform of ScsβA, and a 200bp 3'UTR fragment of the testis isoform of ScsβA surround a tripartite construct comprising: i) the N-terminal region of ScsβG, ii) the central Cul3/Klhl10 interacting region (ckr) of ScsβA, and iii) the C-terminal region of ScsβA with a Tag:HA tag. (Termed hybrid construct VI.)
ScsβA::ScsβGVI.Tag:HA is a non-suppressor of sterile phenotype of ScsβACC06238/Df(3R)osk
ScsβA::ScsβGVI.Tag:HA is a non-suppressor of waste bag phenotype of ScsβACC06238/Df(3R)osk
ScsβA::ScsβGVI.Tag:HA is a non-suppressor of cystic bulge phenotype of ScsβACC06238/Df(3R)osk
Expression of skap::SucbVI.T:Ivir\HA1 fails to rescue the sterility and sperm individualization defects of skapCC06238/Df(3R)osk mutants.