FlyBase Gene Report: Dmel\ScsβA

General Information

Symbol

Dmel\ScsβA

Species

D. melanogaster

Name

Succinyl-coenzyme A synthetase β subunit, ADP-forming

Annotation Symbol

CG11963

Feature Type

protein_coding_gene

FlyBase ID

FBgn0037643

Gene Model Status

Current

Stock Availability

10 publicly available

Enzyme Name (EC)

succinate--CoA ligase (ADP-forming) (6.2.1.5)

Gene Summary

Succinyl-coenzyme A synthetase β subunit, ADP-forming (ScsβA) encodes the ADP-binding subunit of the heterodimeric mitochondrial succinate-CoA ligase complex that catalyzes the fifth step of the tricarboxylic acid (TCA) cycle. The complex hydrolyzes succinyl-CoA into succinate and CoA, coupled to the synthesis of ATP. [Date last reviewed: 2025-03-27] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

skap, SkpA Associated Protein, A-Sβ

Key Links

Genomic Location

Cytogenetic map

85B7-85B7

Sequence location

3R:8,938,724..8,943,027 [+]

Recombination map

3-48

RefSeq locus

NT_033777 REGION:8938724..8943027

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables cysteine-type endopeptidase activator activity involved in apoptotic process

inferred from direct assay

(Aram et al., 2016)

contributes_to succinate-CoA ligase activity

inferred from mutant phenotype

(Quan et al., 2017)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR011761, InterPro:IPR013815

(InterPro Project Members, 2004-)

enables catalytic activity

inferred from electronic annotation with InterPro:IPR005811

(InterPro Project Members, 2004-)

enables metal ion binding

inferred from electronic annotation with InterPro:IPR011761

(InterPro Project Members, 2004-)

enables succinate-CoA ligase (ADP-forming) activity

inferred from sequence or structural similarity with UniProtKB:F1LM47

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000212531

(GO Reference Genome Project, 2011-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in sperm individualization

inferred from mutant phenotype

(Aram et al., 2016)

involved_in tricarboxylic acid cycle

inferred from mutant phenotype

(Quan et al., 2017)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in succinyl-CoA metabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000212531

(GO Reference Genome Project, 2011-)

involved_in tricarboxylic acid cycle

inferred from biological aspect of ancestor with PANTHER:PTN000212531

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR005809, InterPro:IPR034723

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:F1LM47

(Gene Ontology Curators, 2002-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

part_of Cul3-RING ubiquitin ligase complex

inferred from direct assay

(Aram et al., 2016)

located_in major mitochondrial derivative

inferred from direct assay

(Laurinyecz et al., 2019)

located_in mitochondrion

inferred from high throughput direct assay

(Tan et al., 2009)

inferred from direct assay

(Aram et al., 2016)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in mitochondrion

inferred from biological aspect of ancestor with PANTHER:PTN000212530

(GO Reference Genome Project, 2011-)

located_in mitochondrion

inferred from electronic annotation with InterPro:IPR034723

(InterPro Project Members, 2004-)

part_of succinate-CoA ligase complex (ADP-forming)

non-traceable author statement

(Aram et al., 2016)

inferred from sequence or structural similarity with UniProtKB:F1LM47

(Gene Ontology Curators, 2002-)

part_of succinate-CoA ligase complex

inferred from biological aspect of ancestor with PANTHER:PTN000212531

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

SUCCINYL-COA SYNTHETASES

SUCCINATE-COA LIGASE (ADP/ATP) COMPLEX

Pathway (FlyBase)

TRICARBOXYLIC ACID (TCA) CYCLE

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

succinate-CoA ligase (ADP-forming) activity

succinate + ATP + CoA = succinyl-CoA + ADP + phosphate (6.2.1.5)

RHEA 17661:

Summaries

Gene Snapshot

Gene Group (FlyBase)

SUCCINYL-COA SYNTHETASES -: Succinyl-CoA synthetases are mitochondrial enzymes that catalyze the reversible conversion of succinyl-CoA to succinate in the only step of the tricarboxylic acid cycle coupled with substrate-level phosphorylation of either ADP or GDP. In eukaryotes, the functional enzyme is a heterodimeric complex composed of a single alpha subunit and one of two isoforms of a beta subunit that determines the substrate specificity, resulting in an ATP- or GTP-generating enzyme. (Adapted from PMID:37445899.)
SUCCINATE-COA LIGASE (ADP/ATP) COMPLEX -: The succinate-CoA ligase (ADP/ATP) complex is a mitochondrial enzyme that catalyzes the reversible conversion of succinyl-CoA to succinate in the only step of the tricarboxylic acid cycle coupled with substrate-level phosphorylation of ADP. In eukaryotes, the functional enzyme is a heterodimeric complex composed of a single alpha subunit (that is shared with the GTP-forming complex) and a beta subunit that determines the ADP substrate specificity. (Adapted from PMID:37445899.)

Pathway (FlyBase)

TRICARBOXYLIC ACID (TCA) CYCLE -: The tricarboxylic acid (TCA) cycle is a central metabolic process that occurs in mitochondria. It consists of a cyclic series of chemical reactions that harness high-energy electrons from nutrients to produce energy (ATP). Each turn of the TCA cycle oxidizes one acetyl-CoA to two CO2 molecules and yields one GTP or ATP and 6 electrons in the form of four reducing equivalents (three NADH and one FADH2). Then, these electron carriers transfer their electrons onto the electron transport chain (ETC) complexes to generate ATP in a process known as oxidative phosphorylation (OXPHOS). The TCA cycle intermediates can also be used as precursors for the biosynthesis of amino acids, fatty acids and porphyrins and for gluconeogenesis. (Adapted from PMID:36581208).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ScsβA for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q95U38)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Gene model reviewed during 6.04

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ScsβA-RA

FBtr0081957

NM_141589

1736

502

ScsβA-RB

FBtr0113206

NM_001104243

1764

502

ScsβA-RC

FBtr0301786

NM_001170087

1767

503

ScsβA-RD

FBtr0301787

NM_001170088

1739

503

ScsβA-RG

FBtr0334327

NM_001202269

1901

451

ScsβA-RH

FBtr0334328

NM_001202268

1873

451

ScsβA-RI

FBtr0347567

NM_001316547

1876

452

ScsβA-RJ

FBtr0347568

NM_001316546

1904

452

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

ScsβA-PA

FBpp0081437

54.8

502

9.05

FBpp0112118

54.8

502

9.05

FBpp0291000

54.9

503

8.95

FBpp0291001

54.9

503

8.95

FBpp0306436

49.0

451

6.80

FBpp0306437

49.0

451

6.80

FBpp0312599

49.1

452

6.79

FBpp0312600

49.1

452

6.79

A0A126GUR6

NP_001303475

ALI30547

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

502 aa isoforms: ScsβA-PA, ScsβA-PB

503 aa isoforms: ScsβA-PC, ScsβA-PD

452 aa isoforms: ScsβA-PI, ScsβA-PJ

451 aa isoforms: ScsβA-PG, ScsβA-PH

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ScsβA using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.34

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval neuroblast | punctate

(Morciano et al., 2009)

spermatogenesis & adult stage | male

spermatid

• mitochondrion

(Aram et al., 2016)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010, Dorus et al., 2006)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

ScsbetaA immunoreactivity has a punctate distribution in the cytosol of larval neuroblasts, where it colocalizes with sea protein.

(Morciano et al., 2009)

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of Cul3-RING ubiquitin ligase complex

inferred from direct assay

(Aram et al., 2016)

located_in major mitochondrial derivative

inferred from direct assay

(Laurinyecz et al., 2019)

located_in mitochondrion

inferred from high throughput direct assay

(Tan et al., 2009)

inferred from direct assay

(Aram et al., 2016)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ScsβA in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ScsβA

View exonic expression by tissue for Dmel\ScsβA

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0037643

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41067/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0037643

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0037643

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0037643

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11963

Flygut - An atlas of the Drosophila adult midgut

FBgn0037643

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ScsβA

Transgenic constructs containing regulatory region of ScsβA

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(3R)Exel6258

(Ryder, 2004.4.26)

Df(3R)osk

(Reveal et al., 2010)

Inferred from location ( 12 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die

ScsβA^CC06238

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ScsβA^GD7441

viable, with Scer\GAL4^Mef2.PR

ScsβA^GD7441

viable, with Scer\GAL4^pnr-MD237

ScsβA^GD7441

viable, with Scer\GAL4^tin.CΔ4

ScsβA^GD7441

Sterility

Allele

male sterile, with Scer\GAL4^nanos.PG

ScsβA^HMC03847

sterile (with Df(3R)osk)

ScsβA^CC06238

Other Phenotypes

Allele

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

ScsβA^GD7441

Phenotype manifest in

Allele

cystic bulge (with Df(3R)osk)

ScsβA^CC06238

waste bag (with Df(3R)osk)

ScsβA^CC06238

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (3)

Hsap\SUCLA2

12 of 14

Yes

Hsap\SUCLG2

5 of 14

Hsap\ACLY

2 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (3)

Rnor\Sucla2

13 of 14

Yes

Rnor\Suclg2

5 of 14

Rnor\Acly

2 of 14

Mus musculus (laboratory mouse) (3)

Mmus\Sucla2

13 of 14

Yes

Mmus\Suclg2

4 of 14

Mmus\Acly

2 of 14

Xenopus tropicalis (Western clawed frog) (2)

Xtro\sucla2

8 of 13

Yes

Xtro\suclg2

4 of 13

Danio rerio (Zebrafish) (4)

Drer\sucla2

13 of 14

Yes

Drer\suclg2

5 of 14

Drer\aclyb

2 of 14

Drer\aclya

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\suca-1

13 of 14

Yes

Cele\sucg-1

5 of 14

Cele\acly-1

2 of 14

Cele\acly-2

2 of 14

Anopheles gambiae (African malaria mosquito) (4)

Agam\AgaP_AGAP004744

12 of 12

Yes

Agam\AgaP_AGAP004352

4 of 12

Agam\AgaP_AGAP010156

2 of 12

Agam\AgaP_AGAP010155

1 of 12

Arabidopsis thaliana (thale-cress) (6)

Atha\AT2G20420

12 of 13

Yes

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\LSC2

12 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\SPCC1620.08

11 of 12

Yes

Spom\SPAC22A12.16

2 of 12

Spom\SPBC1703.07

1 of 12

Escherichia coli (enterobacterium) (2)

Ecol\sucC

10 of 11

Yes

Ecol\patZ

1 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:ScsβA. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (2)

ScsβG

9 of 13

Acly

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

SUCLA2; succinate-CoA ligase ADP-forming subunit beta

model of mitochondrial DNA depletion syndrome 5

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SUCLA2; succinate-CoA ligase ADP-forming subunit beta

12 of 14

603921

MITOCHONDRIAL DNA DEPLETION SYNDROME 5 (ENCEPHALOMYOPATHIC WITH OR WITHOUT METHYLMALONIC ACIDURIA); MTDPS5

mitochondrial DNA depletion syndrome 5

SUCLG2; succinate-CoA ligase GDP-forming subunit beta

5 of 14

603922

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

ScsβA - ATPsynγ

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Akt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

ScsβA - Arl8

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - CCT3

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - CCT6

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - CCT8

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - CG1371

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - CG1416

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - CG5854

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Cnx99A

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - CtsL1

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Cul3

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Aram et al., 2016)

ScsβA - Cyp12d1-p

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Droj2

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Dsor1

anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting

(Friedman et al., 2011, Friedman et al., 2011)

ScsβA - Fatp1

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Gfat2

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Gp93

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Hel25E

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Idh3a

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - KCNQ

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Gao et al., 2008)

ScsβA - Kr-h2

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Ldh

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Mcad

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Mec2

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Nfs1

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Ost1

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Ost48

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - OstΔ

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Pdk1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

ScsβA - Pp2A-29B

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Pten

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

ScsβA - Rab1

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Rab11

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Rab2

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Rab5

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Raf

anti tag coimmunoprecipitation, anti tag western blot

(Friedman et al., 2011)

ScsβA - RpS5a

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Rpt1

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - S6k

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

ScsβA - S6kII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

ScsβA - SERCA

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - ScsβG

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - SerRS

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - SkpA

anti bait coimmunoprecipitation, western blot

(Hughes et al., 2008)

ScsβA - Tapδ

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - Tspo

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - UQCR-6.4

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - UQCR-C2

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - cathD

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

ScsβA - klhl10

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Aram et al., 2016)

ScsβA - lic

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - mEFTu1

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

ScsβA - soti

anti tag coimmunoprecipitation, anti tag western blot

(Aram et al., 2016)

ScsβA - wal

experimental knowledge based

(Guruharsha et al., 2011)

ScsβA - βCOP

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

ScsβA

suppressible

ScsβA::ScsβG

(Aram et al., 2016)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

klhl10

enhanceable

ScsβA

(Aram et al., 2016)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

66236

DroID - A comprehensive database of gene and protein interactions.

FBgn0037643

MIST (protein-protein) - An integrated Molecular Interaction Database

41067

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

TRICARBOXYLIC ACID (TCA) CYCLE

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

TCA cycle III (animals)

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Citric acid cycle (TCA cycle)

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Citric acid cycle (TCA cycle)

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-48

Cytogenetic map

85B7-85B7

Sequence location

3R:8,938,724..8,943,027 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

85B7-85B7

Limits computationally determined from genome sequence between P{lacW}l(3)L4740^L4740 and P{EP}D1^EP473

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-48

(FlyBase, 2016)

3-45.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

56249

y¹ w^*; Mi{MIC}ScsβA^MI10794

30175

w¹¹¹⁸; P{EP}ScsβA^G6799/TM6C, Sb¹

68138

y¹ sc^* v¹ sev²¹; P{TOE.GS00535}attP40

79401

y¹ sc^* v¹ sev²¹; P{TKO.GS00805}attP40

55168

y¹ sc^* v¹ sev²¹; P{TRiP.HMC03847}attP40

51576

y¹ w^*; P{PTT-GC}ScsβA^CC06238/TM3, Ser¹ Sb¹

77813

y¹ w^*; Mi{Trojan-GAL4.0}ScsβA^{MI10794-TG4.0}/TM3, Sb¹ Ser¹

11963R-3

v105350

v50300

w¹¹¹⁸; P{GD16946}v50300/CyO

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (138)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH10480

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0037643

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0037643

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Hughes et al., 2008)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: skap CG11963

(Hughes et al., 2008)

Source for identity of: ScsβA skap

(Arama et al., 2018.5.3)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

A-Sβ

(Nakajima and Kuranaga, 2017, Aram et al., 2016)

CGI 1963

(Robinson et al., 2010)

FBgn0037643

(Ye and Li, 2016)

ScsbetaA

(Tan et al., 2025)

ScsβA

(François et al., 2023, Palozzi et al., 2022, Meltzer et al., 2019)

SkAP

(Hughes et al., 2008)

Skap

(Galletta et al., 2023, Tsakiri et al., 2019, Aram et al., 2016, Friedman et al., 2011, Morciano et al., 2009)

skap

(Meyer et al., 2024, González Morales et al., 2023, Pang et al., 2021, Laurinyecz et al., 2019, Park et al., 2018, Transgenic RNAi Project members, 2017-, Gene Disruption Project members, 2016-, Choo et al., 2015, Dent et al., 2015, Kwon et al., 2013, Malone et al., 2012, Friedman et al., 2011, Wasbrough et al., 2010, Hughes et al., 2008)

Name Synonyms

ATP-specific SCSβ

(Aram et al., 2016)

ATP-specific form of the succinyl-CoA synthetase β subunit

(Aram et al., 2016)

CG11963

(Zhou et al., 2008)

SkpA Associated Protein

(Morciano et al., 2009, Hughes et al., 2008)

SkpA-associated protein

(Aram et al., 2016)

Succinyl-CoA ligase

(Müller-Eigner et al., 2024)

Succinyl-coenzyme A synthetase β subunit, ADP-forming

skpA associated protein

skpA-associated protein

(Laslo et al., 2013)

succinyl CoA synthetase

(Park et al., 2018)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 75 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

41067

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q95U38

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q95U38

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0037643

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41067/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0037643

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0037643

FlyMine - An integrated database for Drosophila genomics

FBgn0037643

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG11963

MARRVEL_MODEL - MARRVEL (model organism gene)

41067

Linkouts

BioGRID - A database of protein and genetic interactions.

66236

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0037643

DroID - A comprehensive database of gene and protein interactions.

FBgn0037643

DRSC - Results frm RNAi screens

FBgn0037643

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0037643

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0037643

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

TCA cycle III (animals)

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11963

Flygut - An atlas of the Drosophila adult midgut

FBgn0037643

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0037643

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013859

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

41067

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Citric acid cycle (TCA cycle)

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Citric acid cycle (TCA cycle)

References (89)

The following external sites may use different assemblies or annotations than FlyBase.

1.34

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