FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
ScsβA, klhl10
FlyBase ID
FBig0000104637
Interaction Network
Interactions Browser links
ScsβA network
klhl10 network
Reported Interactions
FBrf0231856-10.coIP.WB
Reference
Aram et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
klhl10 

Klhl10

bait

FLAG tag

ScsβA 

A-Sβ

prey

HA tag

ScsβA 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ScsβA 
Cul3-Klhl10 interacting region
sufficient binding region
aa 306-452

coordinates relative to skap-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Aram et al., 2016)
FBrf0231856-2.Y2H
Reference
Aram et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
klhl10 

Klhl10

bait

fused to GAL4 DNA-binding domain

ScsβA 

A-Sβ

prey

fused to GAL4 activation domain

ScsβA 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ScsβA 
Cul3-Klhl10 interacting region
sufficient binding region
aa 306-452

coordinates relative to skap-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Aram et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Aram et al., 2016)
FBrf0231856-3.coIP.WB
Reference
Aram et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
klhl10 

Klhl10

bait

FLAG tag

ScsβA 

A-Sβ

prey

HA tag

ScsβA 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ScsβA 
Cul3-Klhl10 interacting region
sufficient binding region
aa 306-452

coordinates relative to skap-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Aram et al., 2016)
FBrf0231856-7.coIP.WB
Reference
Aram et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ScsβA 

A-Sβ

bait

HA tag

ScsβA 
klhl10 

Klhl10

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ScsβA 
Cul3-Klhl10 interacting region
sufficient binding region
aa 306-452

coordinates relative to skap-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Aram et al., 2016)

Binding of klhl10 to skap is not affected by cotransfection of Cul3.

(Aram et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)