FlyBase Allele Report: Dmel\ScsβA[CC06238]

General Information

Symbol

Dmel\ScsβA^CC06238

Species

D. melanogaster

Name

FlyBase ID

FBal0211619

Feature type

allele

Associated gene

Dmel\ScsβA

Associated Insertion(s)

P{PTT-GC}ScsβA^CC06238

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Buszczak et al., 2007)

Progenitor genotype

Associated Insertion(s)

P{PTT-GC}ScsβA^CC06238

Cytology

Description

Insertion within an intron located between two coding exons, in the appropriate predicted reading frame to fuse the GFP exon in the inserted construct to the endogenous protein.

(Buszczak et al., 2007)

Allele components

Component

Use(s)

Inserted element

P{PTT-GC}

protein trap

Tagged with

EGFP

green fluorescent protein

Tag:polyHis

epitope tag

purification tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:8,938,982..8,938,982 [+]

(Aram et al., 2016, Buszczak et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die

(Buszczak et al., 2007)

sterile (with Df(3R)osk)

(Aram et al., 2016)

Phenotype Manifest In

cystic bulge (with Df(3R)osk)

(Aram et al., 2016)

waste bag (with Df(3R)osk)

(Aram et al., 2016)

Detailed Description

Statement

Reference

skap^CC06238/Df(3R)osk mutant males are sterile, and are defective in sperm individualization, as detected by absence of cystic bulges and waste bags in the testis.

(Aram et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ScsβA^CC06238/Df(3R)osk has sterile phenotype, suppressible by ScsβA::ScsβG^V.Tag:HA

(Aram et al., 2016)

NOT suppressed by

Statement

Reference

ScsβA^CC06238/Df(3R)osk has sterile phenotype, non-suppressible by ScsβG^{ScsβA.Tag:HA}

(Aram et al., 2016)

ScsβA^CC06238/Df(3R)osk has sterile phenotype, non-suppressible by ScsβA::ScsβG^VI.Tag:HA

(Aram et al., 2016)

ScsβA^CC06238/Df(3R)osk has sterile phenotype, non-suppressible by ScsβA::ScsβG^VIII.Tag:HA

(Aram et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

ScsβA^CC06238/Df(3R)osk has waste bag phenotype, suppressible by ScsβA::ScsβG^V.Tag:HA

(Aram et al., 2016)

ScsβA^CC06238/Df(3R)osk has cystic bulge phenotype, suppressible by ScsβA::ScsβG^V.Tag:HA

(Aram et al., 2016)

NOT suppressed by

Statement

Reference

ScsβA^CC06238/Df(3R)osk has waste bag phenotype, non-suppressible by ScsβG^{ScsβA.Tag:HA}

(Aram et al., 2016)

ScsβA^CC06238/Df(3R)osk has waste bag phenotype, non-suppressible by ScsβA::ScsβG^VI.Tag:HA

(Aram et al., 2016)

ScsβA^CC06238/Df(3R)osk has waste bag phenotype, non-suppressible by ScsβA::ScsβG^VIII.Tag:HA

(Aram et al., 2016)

ScsβA^CC06238/Df(3R)osk has cystic bulge phenotype, non-suppressible by ScsβG^{ScsβA.Tag:HA}

(Aram et al., 2016)

ScsβA^CC06238/Df(3R)osk has cystic bulge phenotype, non-suppressible by ScsβA::ScsβG^VI.Tag:HA

(Aram et al., 2016)

ScsβA^CC06238/Df(3R)osk has cystic bulge phenotype, non-suppressible by ScsβA::ScsβG^VIII.Tag:HA

(Aram et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Sucb^{skap.T:Ivir\HA1}, skap::Sucb^{VI.T:Ivir\HA1} or skap::Sucb^{VIII.T:Ivir\HA1} fails to rescue the sterility and sperm individualization defects of skap^CC06238/Df(3R)osk mutants.

Expression of skap::Sucb^V.T:Ivir\HA1 fully rescues the sterility and sperm individualization defects of skap^CC06238/Df(3R)osk mutants.

(Aram et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ScsβA^CC06238/Df(3R)osk is rescued by ScsβA^T.Tag:HA

(Aram et al., 2016)

ScsβA^CC06238/Df(3R)osk is rescued by ScsβA^S.Tag:HA

(Aram et al., 2016)

Comments

Expression of skap^T.T:Ivir\HA1 or skap^S.T:Ivir\HA1 fully rescues the sterility and sperm individualization defects of skap^CC06238/Df(3R)osk mutants.

(Aram et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51576

y¹ w^*; P{PTT-GC}ScsβA^CC06238/TM3, Ser¹ Sb¹

Notes on Origin

Discoverer

Comments

Protein trap line.

(Buszczak et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

A-Sβ^cc

(Aram et al., 2016)

CG11963^CC06238

ScsβA^CC06238

skap^CC06238

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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