A 500bp fragment containing the 5'UTR and upstream promoter region of the testis isoform of ScsβA, and a 200bp 3'UTR fragment of the testis isoform of ScsβA surround a tripartite construct comprising: i) the N-terminal region of ScsβA, ii) the central Cul3/Klhl10 interacting region (ckr) of ScsβG, and iii) the C-terminal region of ScsβA with a Tag:HA tag. (Termed hybrid construct V.)
ScsβA::ScsβGV.Tag:HA is a suppressor of sterile phenotype of ScsβACC06238/Df(3R)osk
ScsβA::ScsβGV.Tag:HA is a suppressor of waste bag phenotype of ScsβACC06238/Df(3R)osk
ScsβA::ScsβGV.Tag:HA is a suppressor of cystic bulge phenotype of ScsβACC06238/Df(3R)osk
Expression of skap::SucbV.T:Ivir\HA1 fully rescues the sterility and sperm individualization defects of skapCC06238/Df(3R)osk mutants.