FlyBase Allele Report: Zzzz\CAG[100.UAS.DsRed2]

General Information

Symbol

Zzzz\CAG^{100.UAS.DsRed2}

Species

a. artificial

Name

FlyBase ID

FBal0319634

Feature type

allele

Associated gene

Zzzz\CAG

Associated Insertion(s)

Carried in Construct

P{UAS-DsRed2.CAG100}

Also Known As

UAS-DsRed_CAG100

Transgenic product class

trinucleotide_repeat_expansion

(Li et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

trinucleotide_repeat_expansion

(Li et al., 2008)

Mutagen

in vitro construct

(Li et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-DsRed2.CAG100}

Cytology

Description

UASt regulatory sequences drive expression of DsRed2 followed by a 3'UTR containing untranslated CAG repeats (repeat length of 100).

(Li et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Zzzz\CAG

Tagged with

DsRed2

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Peng et al., 2021, Zhang et al., 2018, Tsoi et al., 2011)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of neurodegenerative disease

is exacerbated by U2af50^RNAi.UAS.cUa

(Tsoi et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

brain | adult stage | progressive, with Scer\GAL4^elav-C155

(Li et al., 2008)

eye | adult stage, with Scer\GAL4^GMR.PU

(Wong et al., 2014)

retina, with Scer\GAL4^GMR.PF

(Li et al., 2008)

rhabdomere of eye photoreceptor cell, with Scer\GAL4^GMR.PF

(Tsoi et al., 2011)

Detailed Description

Statement

Reference

Expression of Zzzz\CAG^{100.Scer\UAS.T:Disc\RFP-DsRed2} under the control of Scer\GAL4^GMR.PU results in a rough eye phenotype.

(Wong et al., 2014)

Co-expression of Disc\RFP^{CAG100.DsRed2.Scer\UAS} with Disc\RFP^{(CTG)130.Scer\UAS} in the muscle, under the control of Scer\GAL4^how-24B, results in developmental lethality.

Expression of Disc\RFP^{CAG100.DsRed2.Scer\UAS} in the muscle, under the control of Scer\GAL4^how-24B, does not generate a phenotype.

(Yu et al., 2011)

One day old flies expressing four copies of Disc\RFP^{CAG100.DsRed2.Scer\UAS} in the eye under the control of Scer\GAL4^GMR.PF show internal loss of retinal tissue, with holes within the retina. Normal brain morphology is seen in one day old flies expressing five copies of Disc\RFP^{CAG100.DsRed2.Scer\UAS} in neurons under the control of Scer\GAL4^elav-C155, but flies undergo striking degeneration of the brain over time and have vacuoles in the brain at 35 days. The flies die early with progressive loss of climbing ability compared to controls.

(Li et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Zzzz\CAG^{100.UAS.DsRed2} has abnormal neuroanatomy phenotype, enhanceable by U2af50^RNAi.UAS.cUa/Scer\GAL4^GMR.PF

(Tsoi et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^how-24B, Zzzz\CAG^{100.UAS.DsRed2}, Zzzz\CTG^{130.UAS.DsRed2} has increased mortality during development phenotype, suppressible by Dcr-2^L811fsX

(Yu et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Zzzz\CAG^{100.UAS.DsRed2} has rhabdomere of eye photoreceptor cell phenotype, enhanceable by U2af50^RNAi.UAS.cUa/Scer\GAL4^GMR.PF

(Tsoi et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

A Dcr-2^L811fsX homozygous mutant background suppresses the developmental lethality associated with expression of Disc\RFP^{(CTG)130.Scer\UAS} and Disc\RFP^{CAG100.DsRed2.Scer\UAS} in the developing muscle (under the control of Scer\GAL4^how-24B)

(Yu et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Disc\RFP^{CAG100.DsRed2.Scer\UAS}

Zzzz\CAG^{100.Scer\UAS.T:Disc\RFP-DsRed2}

Zzzz\CAG^{100.UAS.DsRed2}

Name Synonyms

Secondary FlyBase IDs

FBal0319579
FBal0283319

References (10)

Report Sections

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