FlyBase Allele Report: Dmel\rictor[77A]

General Information

Symbol

Dmel\rictor^77A

Species

D. melanogaster

Name

FlyBase ID

FBal0319806

Feature type

allele

Associated gene

Dmel\rictor

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Jevtov et al., 2015)

Progenitor genotype

P{EPgy2}rictor^EY08986

(Jevtov et al., 2015)

Cytology

Description

Approximately 1.4kb deletion that removes rictor exons 2 and 3. This generates a premature stop codon after 58 amino acid residues. Generated by imprecise excision of P{EPgy2}rictor^EY08986.

(Jevtov et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:19,258,539..19,259,939 [-]

Comment:

Approximate boundaries of a ~1.4kb deletion resulting from the imprecise excision of P{EPgy2}rictor^EY08986 that removes rictor exons 2 and 3.

(Jevtov et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal heat stress response | adult stage

(Jevtov et al., 2015)

abnormal heat stress response | larval stage

(Jevtov et al., 2015)

decreased body size

(Jevtov et al., 2015)

decreased body size (with rictor^305A)

(Jevtov et al., 2015)

viable

(Jevtov et al., 2015)

viable (with rictor^305A)

(Jevtov et al., 2015)

Phenotype Manifest In

cytoplasmic stress granule | larval stage | heat sensitive

(Jevtov et al., 2015)

embryonic/larval hemocyte | larval stage | heat sensitive

(Jevtov et al., 2015)

imaginal disc | heat sensitive

(Jevtov et al., 2015)

Detailed Description

Statement

Reference

rictor^77A/rictor^77A, rictor^77A/rictor^305A and hemizygous rictor^77A flies are viable with no obvious morphological defects, but are slightly reduced in size.

rictor^77A mutant adult males are highly sensitive to heat stress as compared with controls, becoming sluggish and paralyzed upon heat exposure at 37[o]C, and are able to recover once returned to normal temperature. These flies do not exhibit sensitivity to oxidative stress or starvation as compared to controls. rictor^77A mutant larvae exhibit increased heat sensitivity, with absent or reduced mobility as compared to controls upon heat exposure at 37[o]C. In contrast to controls, mutant larvae fail to form stress granules in their imaginal discs or hemocytes 2 hours after heat exposure, but stress granule formation in the brain appears unaffected.

(Jevtov et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

rictor^77A is rescued by Scer\GAL4^Tub.PU/rictor^UAS.Tag:HA

(Jevtov et al., 2015)

Comments

Expression of rictor^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^tub.PU rescues the heat sensitivity of rictor^77A mutant flies.

(Jevtov et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rictor^77A

(Jevtov et al., 2015)

rictor^77A

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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