FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\NbrD435A.E437A
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General Information
Symbol
Dmel\NbrD435A.E437A
Species
D. melanogaster
Name
FlyBase ID
FBal0319833
Feature type
allele
Associated gene
Dmel\Nbr
Associated Insertion(s)
Carried in Construct
P{Nbr.D435A,E437A}
Key Links
Transgenic product class
missense_variant
(Feltzin et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Feltzin et al., 2015)
Mutagen
in vitro construct
(Feltzin et al., 2015)
Progenitor genotype
Carried in construct
P{Nbr.D435A,E437A}
Cytology
Description

Genomic transgene of Nbr, including ~650 bp upstream and ~150 bp downstream of the Nbr ORF, which is rendered catalytically dead through D435A and E437A mutations in the exonuclease domain.

(Feltzin et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
Nbr
Encoded product / tool
Nbr
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      NbrD435A.E437A fails to rescue Nbrf02257/Df(2L)BSC312

      (Feltzin et al., 2015)
      Comments

      Nbr+tFa but not NbrD435A.E437A rescues the progressive held up wing, brain vacuolization and locomotor phenotypes in Nbrf02257/Df(2L)BSC312 flies; in terms of locomotor defects, NbrD435A.E437A leads to significant defects compared to controls at all ages (including the first time point tested, day 2).

      (Feltzin et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      NbrD435A.E437A
      Name Synonyms
      Secondary FlyBase IDs
        References (1)