FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\U2A1.W
Open Close
General Information
Symbol
Dmel\U2A1.W
Species
D. melanogaster
Name
FlyBase ID
FBal0319834
Feature type
allele
Associated gene
Dmel\U2A
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Progenitor genotype
    Cytology
    Description

    7bp deletion in exon 1 (nucleotides 253-259 relative to the start codon are removed). This results in a frameshift and loss of the conserved leucine-rich repeat C-terminal and U2A domains.

    (Wu et al., 2016)
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    deletion
    2R:7,675,977..7,675,983 [-]
    Comment:

    A 7 bp deletion in the U2A coding sequence within the leucine-rich repeat causes a frameshift.

    (Wu et al., 2016)
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        Using U2Ahs.PW in combination with heat shock to rescue lethality (then switching off rescue during adulthood to study mutant phenotypes), the following phenotypes were observed in U2A1.W/U2A1.W flies: males are sterile and lack mature sperm, the apical region of the testes contains many germ cells resembling early germ cells (likely spermatogonial cells) but frequently lack differentiating germ cell cysts. Mutants have a small population of germline stem cells around the hub, similar to wild type. Mutant testes contain many cysts with more than 16 spermatogonia and only 25% of cysts progress to the spermatocyte state (many of which have over 32 spermatocytes per cyst) and many germ cell cysts are mitotic, suggesting excessive proliferation of spermatogonia that fail to enter meiosis and differentiation.

        (Wu et al., 2016)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Suppressed by
        Statement
        Reference

        U2A1.W has lethal phenotype, suppressible by Hsap\SNRPA1UASp.cWa/Scer\GAL4αTub84B.PL

        (Wu et al., 2016)
        Phenotype Manifest In
        Additional Comments
        Genetic Interactions
        Statement
        Reference
        Xenogenetic Interactions
        Statement
        Reference

        Expression of Hsap\SNRPA1Scer\UAS.P\T.cWa driven by Scer\GAL4αTub84B.PL suppresses lethality in U2A1.W/U2A1.W mutants: flies are fertile and have normal testis morphology and spermatogonial development.

        (Wu et al., 2016)
        Complementation and Rescue Data
        Rescued by

        U2A1.W is rescued by U2Ahs.PW

        (Wu et al., 2016)

        U2A1.W is rescued by U2AUASp.cWa/Scer\GAL4αTub84B.PL

        (Wu et al., 2016)
        Comments

        Continuous heat shock treatment with U2Ahs.PW rescues lethality of U2A1.W/U2A1.W, allowing survival to adulthood.

        Expression of U2AScer\UAS.P\T.cWa driven by Scer\GAL4αTub84B.PL rescues sterility in U2A1.W/U2A1.W males.

        (Wu et al., 2016)
        Images (0)
        Mutant
        Wild-type
        Stocks (0)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (2)
        Reported As
        Symbol Synonym
        U2A1.W
        U2A1
        (Wu et al., 2016)
        Name Synonyms
        Secondary FlyBase IDs
          References (1)