FlyBase Allele Report: Dmel\E(spl)m8-HLH[CK2.UAS.Tag:FLAG]

General Information

Symbol

Dmel\E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}

Species

D. melanogaster

Name

FlyBase ID

FBal0319991

Feature type

allele

Associated gene

Dmel\E(spl)m8-HLH

Associated Insertion(s)

Carried in Construct

PBac{UAS-E(spl)m8-HLH.CK2.FLAG}

Also Known As

UAS-m8^CK2

Key Links

Transgenic product class

missense_variant

(Bivik et al., 2016)

polypeptide_post_translational_processing_variant

(Bivik et al., 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Bivik et al., 2016)

polypeptide_post_translational_processing_variant

(Bivik et al., 2016)

Mutagen

in vitro construct

(Bivik et al., 2016)

Progenitor genotype

Carried in construct

PBac{UAS-E(spl)m8-HLH.CK2.FLAG}

Cytology

Description

UAS regulatory sequences drive expression of a Drosophila codon-optimized E(spl)m8-HLH open reading frame (no 5'UTR or 3' UTR sequences are present) that carries mutations in the CK2 phosphorylation site (three Ser to Ala replacements). The open reading frame is tagged at the N-terminal end with Tag:FLAG.

(Bivik et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

E(spl)m8-HLH

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic neuroblast | embryonic stage 11, with Scer\GAL4^da.G32

(Arefin et al., 2019)

thoracic neuroblast | embryonic stage, with Scer\GAL4^GMR42G10

(Bivik et al., 2016)

Detailed Description

Statement

Reference

At stage 11 expression of E(spl)m8-HLH^{CK2.UAS.Tag:FLAG} under the control of Scer\GAL4^da.G32 leads to a reduction in neuroblast numbers when compared to control embryos.

(Arefin et al., 2019)

Expression of E(spl)m8-HLH^{CK2.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR42G10 in embryos results in significant reduction of thoracic neuroblast daughter cell proliferation at stage 12 (but not stage 14), thoracic neuroblasts at stage 12 (but not stage 14), abdominal neuroblast daughter cell proliferation at stage 12 and 14, but no significant difference in abdominal neuroblast proliferation at stage 12 and 14, and also display reduced cell number of the neuroblast NB5-6T lineage, as compared to controls.

(Bivik et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}, Scer\GAL4^GMR42G10 has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by cas^UAS.cKa/Antp^UAS.cMb, Scer\GAL4^GMR42G10

(Bivik et al., 2016)

NOT Enhanced by

Statement

Reference

E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}, Scer\GAL4^GMR42G10 has abnormal neuroanatomy | embryonic stage phenotype, non-enhanceable by Df(3L)ED225/Df(3L)ED225

(Bivik et al., 2016)

Enhancer of

Statement

Reference

E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}, Scer\GAL4^GMR42G10 is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of Antp^UAS.cMb, Scer\GAL4^GMR42G10, cas^UAS.cKa

(Bivik et al., 2016)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}, Scer\GAL4^GMR42G10 has thoracic neuroblast | embryonic stage phenotype, non-enhanceable by cas^UAS.cKa/Antp^UAS.cMb, Scer\GAL4^GMR42G10

(Bivik et al., 2016)

E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}, Scer\GAL4^GMR42G10 has thoracic neuroblast | embryonic stage phenotype, non-enhanceable by Df(3L)ED225/Df(3L)ED225

(Bivik et al., 2016)

NOT Enhancer of

Statement

Reference

E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}, Scer\GAL4^GMR42G10 is a non-enhancer of thoracic neuroblast | embryonic stage phenotype of Antp^UAS.cMb, Scer\GAL4^GMR42G10, cas^UAS.cKa

(Bivik et al., 2016)

E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}, Scer\GAL4^GMR42G10 is a non-enhancer of abdominal neuroblast | embryonic stage phenotype of Antp^UAS.cMb, Scer\GAL4^GMR42G10, cas^UAS.cKa

(Bivik et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of E(spl)m8-HLH^{CK2.Scer\UAS.T:Zzzz\FLAG}, cas^Scer\UAS.cKa and Antp^Scer\UAS.cMb under the control of Scer\GAL4^GMR42G10 enhances the reduced proliferation phenotype in neuroblast daughter cells, but does not enhance the reduced proliferation of neuroblasts, as compared with embryos expressing E(spl)m8-HLH^{CK2.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR42G10.

Expression of E(spl)m8-HLH^{CK2.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR42G10 in a Df(3L)ED225/Df(3L)ED225 background results in a similar phenotype to embryos expressing E(spl)m8-HLH^{CK2.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR42G10.

(Bivik et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

E(spl)m8-HLH^{CK2.Scer\UAS.T:Zzzz\FLAG}

E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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