FlyBase Allele Report: Dmel\Pfdn2[Δ10]

General Information

Symbol

Dmel\Pfdn2^Δ10

Species

D. melanogaster

Name

FlyBase ID

FBal0320353

Feature type

allele

Associated gene

Dmel\Pfdn2

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Zhang et al., 2016)

Mutagen

P-element activity

(Zhang et al., 2016)

Progenitor genotype

P{EPgy2}Pfdn2^EY06124

(Zhang et al., 2016)

Cytology

Description

Deletion that removes the entire Pfdn2 open reading frame.

(Zhang et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Pfdn2^Δ10 third instar larvae show ectopic neuroblasts in third instar larval brains and an increase in the number of cells in S-phase (assessed by EdU labelling). They also display defects in asymmetric protein segregation in neuroblasts during both metaphase and telophase.

Pfdn2^Δ10/Pfdn2^Δ10 neuroblast (both type I and type II lineages) somatic MARCM clones generate excess neuroblast cells.

Pfdn2^Δ10 third instar larval brain neuroblasts often contain irregular number of centrosomes during mitosis (either more than two or just one - leading to monopolar mitotic spindle) and majority of them fail to assemble astral microtubules during metaphase. Pfdn2^Δ10 larvae also display impaired spindle microtubule regrowth after cold-induced depolarization compared to wild-type.

Pfdn2^Δ10/Pfdn2⁰¹²³⁹ transheterozygotes display increased average number of brain neuroblasts compared to wild-type.

(Zhang et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Pfdn2^Δ10 has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by mgr^G5308

(Zhang et al., 2016)

Pfdn2^Δ10 has increased cell number | third instar larval stage phenotype, enhanceable by pins^P89

(Zhang et al., 2016)

Pfdn2^Δ10 has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by pins^P89

(Zhang et al., 2016)

Suppressed by

Statement

Reference

Pfdn2^Δ10/Pfdn2⁰¹²³⁹ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by aPKC^GD1366/Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

Pfdn2^Δ10/Pfdn2⁰¹²³⁹ has increased cell number | third instar larval stage phenotype, suppressible | partially by aPKC^GD1366/Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

Pfdn2^Δ10/Pfdn2⁰¹²³⁹ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by par-6^GD4048/Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

Pfdn2^Δ10/Pfdn2⁰¹²³⁹ has increased cell number | third instar larval stage phenotype, suppressible | partially by par-6^GD4048/Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

Pfdn2^Δ10, pins^P89 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by pins^UAS.cYa/Scer\GAL4^erm.PU

(Zhang et al., 2016)

Pfdn2^Δ10, pins^P89 has increased cell number | third instar larval stage phenotype, suppressible | partially by pins^UAS.cYa/Scer\GAL4^erm.PU

(Zhang et al., 2016)

Pfdn2^Δ10, pins^P89 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by αTub84B^{Ubi-p63E.GFP(S65C)}

(Zhang et al., 2016)

Pfdn2^Δ10, pins^P89 has increased cell number | third instar larval stage phenotype, suppressible | partially by αTub84B^{Ubi-p63E.GFP(S65C)}

(Zhang et al., 2016)

Enhancer of

Statement

Reference

Pfdn2^Δ10 is an enhancer of abnormal neuroanatomy phenotype of mgr^G5308

(Zhang et al., 2016)

Suppressor of

Statement

Reference

Pfdn2^Δ10 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of pins^P89

(Zhang et al., 2016)

Pfdn2^Δ10 is a suppressor of decreased cell number | third instar larval stage phenotype of pins^P89

(Zhang et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pfdn2^Δ10 has neuroblast | third instar larval stage phenotype, enhanceable by mgr^G5308

(Zhang et al., 2016)

Pfdn2^Δ10 has neuroblast | third instar larval stage phenotype, enhanceable by pins^P89

(Zhang et al., 2016)

Pfdn2^Δ10 has neuroblast | third instar larval stage | increased number phenotype, enhanceable by pins^P89

(Zhang et al., 2016)

Pfdn2^Δ10 has mitotic spindle | third instar larval stage phenotype, enhanceable by pins^P89

(Zhang et al., 2016)

Pfdn2^Δ10 has spindle microtubule | third instar larval stage phenotype, enhanceable by pins^P89

(Zhang et al., 2016)

Pfdn2^Δ10 has mitotic spindle | third instar larval stage phenotype, enhanceable by mgr^G5308

(Zhang et al., 2016)

Pfdn2^Δ10 has centrosome | third instar larval stage phenotype, enhanceable by mgr^G5308

(Zhang et al., 2016)

Pfdn2^Δ10 has spindle microtubule | third instar larval stage phenotype, enhanceable by mgr^G5308

(Zhang et al., 2016)

Suppressed by

Statement

Reference

Pfdn2^Δ10, pins^P89 has neuroblast | third instar larval stage phenotype, suppressible | partially by αTub84B^{Ubi-p63E.GFP(S65C)}

(Zhang et al., 2016)

Pfdn2^Δ10/Pfdn2⁰¹²³⁹ has neuroblast | increased number | third instar larval stage phenotype, suppressible | partially by aPKC^GD1366/Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

Pfdn2^Δ10/Pfdn2⁰¹²³⁹ has neuroblast | increased number | third instar larval stage phenotype, suppressible | partially by par-6^GD4048/Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

Enhancer of

Statement

Reference

Pfdn2^Δ10 is an enhancer of neuroblast | third instar larval stage phenotype of mgr^G5308

(Zhang et al., 2016)

Pfdn2^Δ10 is an enhancer of mitotic spindle | third instar larval stage phenotype of mgr^G5308

(Zhang et al., 2016)

Pfdn2^Δ10 is an enhancer of centrosome | third instar larval stage phenotype of mgr^G5308

(Zhang et al., 2016)

Suppressor of

Statement

Reference

Pfdn2^Δ10 is a suppressor of neuroblast | third instar larval stage phenotype of pins^P89

(Zhang et al., 2016)

Other

Statement

Reference

Pfdn2^Δ10, pins^P89 has intermediate neuronal progenitor | third instar larval stage phenotype

(Zhang et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The microtubule and centrosomal abnormalities (irregular number of centrosomes and spindle defects, including loss of astral microtubules) observed in brain neuroblasts in Pfdn2^Δ10 single mutant and mgr^G5308/Df(3R)Exel6160 third instar larvae are exacerbated further in Pfdn2^Δ10,mgr^G5308 double mutants.

The increased average number of brain neuroblasts (compared to wild-type) seen in Pfdn2^Δ10 mutant third instar larvae is strongly enhanced by combination with pins^P89 although pins^P89 mutants on their own display decreased number of brain neuroblasts compared to wild-type. The defects in asymmetric protein segregation and mitotic spindle structure in neuroblasts observed both in Pfdn2^Δ10 and pins^P89 single mutants are strongly enhanced in Pfdn2^Δ10;pins^P89 double mutants: all neuroblasts show impaired asymmetric protein segregation and divide symmetrically. Symmetric division is observed also in intermediate neural progenitor cells in the double mutants - a phenotype not detected in either of the single mutants.

The dramatically increased total average number of brain neuroblast in third instar larvae characteristic for Pfdn2^Δ10;pins^P89 double mutants can be partially rescued by expression of pins^Scer\UAS.cYa under the control of Scer\GAL4^erm.PU in the double mutant background.

The dramatically increased average number of brain neuroblasts characteristic for Pfdn2^Δ10/Pfdn2⁰¹²³⁹ transheterozygote third instar larvae is partially restored by expression of aPKC^GD1366 under the control of Scer\GAL4^insc-Mz1407 in the mutant background.

The dramatically increased average number of brain neuroblasts characteristic for Pfdn2^Δ10/Pfdn2⁰¹²³⁹ transheterozygote third instar larvae is partially restored by expression of par-6^GD4048 under the control of Scer\GAL4^insc-Mz1407 in the mutant background.

The increased number of brain neuroblast as well as the asymmetric protein segregation defects (observed both during metaphase and telophase) characteristic for Pfdn2^Δ10;pins^P89 double mutants are partially rescued by expression of αTub84B^{Ubi-p63E.T:Avic\GFP-S65C} in the double mutant background.

(Zhang et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Pfdn2^Δ10 is rescued by Scer\GAL4^insc-Mz1407/Pfdn2^UAS.Venus

(Zhang et al., 2016)

Pfdn2^Δ10 is rescued by Pfdn2^UAS.Venus/Scer\GAL4^Tub.PU

(Zhang et al., 2016)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pfdn2^Δ10

pfdn2^Δ10

(Zhang et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (1)

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