FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Pp2B-14Dact.tin.C.Tag:FLAG
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General Information
Symbol
Dmel\Pp2B-14Dact.tin.C.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0320394
Feature type
allele
Associated gene
Dmel\Pp2B-14D
Associated Insertion(s)
Carried in Construct
P{tinC-FLAG-Pp2B-14D.act}
Key Links
Transgenic product class
inframe_deletion
(Lee et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Lee et al., 2014)
Mutagen
in vitro construct
(Lee et al., 2014)
Progenitor genotype
Carried in construct
P{tinC-FLAG-Pp2B-14D.act}
Cytology
Description

A 304bp cardiac-specific tin regulatory region and Hsp70 minimal promoter drives expression of constitutively active Pp2B-14D (lacks the C-terminal portion of the protein, including the autoinhibitory domain) that is tagged at the N-terminal end with Tag:FLAG.

(Lee et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
tin
Encoded product / tool
Pp2B-14D
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cardiomyopathy
      CEA
      (Lee et al., 2014)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  cardiomyopathy
      is ameliorated by Mef2X1
      (Lee et al., 2014)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Pp2B-14Dact.tin.C.T:Zzzz\FLAG in adults results in enlarged end-diastolic dimension of the cardiac chamber and reduced fractional shortening, as compared to controls, and these phenotypes persist with age.

      (Lee et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Pp2B-14Dact.tin.C.Tag:FLAG has adult heart phenotype, suppressible by Mef2[+]/Mef2X1

      (Lee et al., 2014)
      NOT suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Mef2X1/+ rescues the cardiac enlargement and reduced fractional shortening phenotypes seen in flies expressing Pp2B-14Dact.tin.C.T:Zzzz\FLAG.

      Expression of Doc1GD4334, Doc2GD4335, Doc1JF02222 or Doc3JF02223 under the control of Scer\GAL4tin.CΔ4 does not rescue the cardiac enlargement of flies expressing Pp2B-14Dact.tin.C.T:Zzzz\FLAG.

      (Lee et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Pp2B-14Dact.tin.C.T:Zzzz\FLAG
      Pp2B-14Dact.tin.C.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)