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General Information
Symbol
Dmel\Ogadel.1
Species
D. melanogaster
Name
FlyBase ID
FBal0321420
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

Estimated boundaries of a 657 bp deletion reported to start upstream of Oga and remove 171 amino acids of the protein.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Excision of the progenitor insertion (that is inserted 100 bp upstream of the Oga ATG start site), removing 657 bp of the Oga gene including the promoter, first exon, and a portion of the second exon. This is predicted to remove 171 amino acids at the N terminus of the protein sequence, corresponding to over half of the predicted O-GlcNAcase domain including nearly all of the catalytically important residues. Transcript levels are reduced to near zero at the deleted locus, though the rest of the gene is transcribed at levels comparable with wild type - therefore it is possible that a truncated protein containing an intact C-terminal domain, including the pseudo-histone acetyltransferase domain, is still made.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Ogadel.1 homozygous mutants are viable and fertile but the females display semi-penetrant oogenesis defect and lay significantly fewer eggs per day when mated with either Ogadel.1 mutant or wild-type male. The females have smaller degenerate ovaries compared to wild-type.

Ogadel.1/Df(3R)ED10838 as well as Ogadel.1/Df(3R)ED10845 mutant flies show normal fertility.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of OgaScer\UAS.cKa.T:Ivir\HA1 under the control of either Scer\GAL4Act.PU or Scer\GAL4nos.PU rescues the impaired oogenesis as well as defective ovary morphology characteristic for Ogadel.1 homozygous females - the number of eggs laid by Ogadel.1 mutant females ectopically expressing OgaScer\UAS.cKa.T:Ivir\HA1 is even higher than in wild-type.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Ogadel.1
Name Synonyms
Secondary FlyBase IDs
    References (1)