1000bp deletion ( 3R:24192426..24193426 , release 6 genome) within jnj, removing the 5'UTR and the first three coding exons.
1001 bp deletion resulting from the imprecise excision of P{EP}EP545 reported to mao to 3R:24192426..24193426 , R6 coordinates.
Eggs from jnjΔ35/jnjΔ35 mothers exhibit a significant reduction in hatching rate, as compared to wild type, and a subset of these embryos arrest at embryonic stage 2, but both of these phenotypes are suppressible when reared at 18[o]C instead of 25[o]C. Embryos also exhibit developmental delay and nuclear abnormalities that are not suppressible by rearing at 18[o]C, including nuclear degeneration and erroneous nuclear divisions; at stages 4-5 exhibit a "nuclear fallout" phenotype wherein faulty nuclei are actively removed into the yolk; after cleavage cycles these embryos exhibit frequent anaphase-bridge formation and gaps in the embryonic cortex; but no abnormalities in eggshell patterning are observed.
jnjΔ35/jnjΔ35 mutant females exhibit an increase in X chromosome, but not autosome, non-disjunction during meiosis, and this phenotype is suppressible by rearing flies at 18[o]C instead of 25[o]C; no difference in crossover rate is seen in these mutants, as compared to controls. Germaria in jnjΔ35/jnjΔ35 mutant females exhibit similar levels of DNA double stranded breaks compared to wild type, but these are insufficiently repaired in mutants, and this phenotype is suppressed when rearing flies at 18[o]C instead of 25[o]C. Ovaries of jnjΔ35/jnjΔ35 mutant females exhibit increased pachytene arrest and delayed karyosome dynamics, as compared to wild type.
SMC6Δ35 has abnormal meiotic cell cycle | female | temperature conditional phenotype, suppressible by mei-W682-0949/mei-W682-0949
SMC6Δ35 has nucleus | maternal effect | embryonic stage phenotype, suppressible by lok[+]/lokp6
SMC6Δ35 has ovary phenotype, suppressible by mei-W682-0949/mei-W682-0949
SMC6Δ35 has karyosome phenotype, suppressible by mei-W682-0949/mei-W682-0949
Embryos at stages 4-5 from lokp6/+, jnjΔ35/jnjΔ35 double mutant mothers exhibit reduced nuclear fallout as compared to those from jnjΔ35/jnjΔ35 single mutant mothers.
mei-W682-0949/mei-W682-0949, jnjΔ35/jnjΔ35 double mutants exhibit suppression of the pachytene arrest and delay in karyosome dynamics, as compared to jnjΔ35/jnjΔ35 single mutants.
SMC6Δ35 is partially rescued by SMC6Tag:HA
Expression of jnjT:Ivir\HA1 partially rescues the lethality, developmental arrest, nuclear fallout, and anaphase-bridge formation phenotypes exhibited by embryos from jnjΔ35/jnjΔ35 mothers, and partially rescues the X chromosome non-disjunction, impaired double strand break repair, pachytene arrest and delayed karyosome dynamics phenotypes exhibited in jnjΔ35/jnjΔ35 female gonads.