FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\AckKD.UAS.Tag:V5
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General Information
Symbol
Dmel\AckKD.UAS.Tag:V5
Species
D. melanogaster
Name
FlyBase ID
FBal0321754
Feature type
allele
Associated gene
Dmel\Ack
Associated Insertion(s)
Carried in Construct
P{UAS-Ack.V5.KD}
Key Links
Transgenic product class
missense_variant
(Hu et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Hu et al., 2016)
Mutagen
in vitro construct
(Hu et al., 2016)
Progenitor genotype
Carried in construct
P{UAS-Ack.V5.KD}
Cytology
Description

UASt regulatory sequences drive expression of a kinase-dead form of Ack (contains the amino acid replacement K156R). The open reading frame is tagged with Tag:V5.

(Hu et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Ack
Tagged with
Tag:V5
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of AckKD.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4Bx-MS1096 results in significantly smaller adult wings, while expression under Scer\GAL4GMR.PU has no significant effect on adult eye size relative to controls.

      The eye overgrowth observed in adult flies expressing either ykiScer\UAS.cZa or ykiS168A.Scer\UAS.cRa.T:Hsap\MYC under the control of Scer\GAL4GMR.PU is exacerbated further by co-expression of AckScer\UAS.T:SV5\V5 (which on its own also promotes eye growth) but not AckKD.Scer\UAS.T:SV5\V5.

      Expression of AckScer\UAS.T:SV5\V5 (but not AckKD.Scer\UAS.T:SV5\V5) under the control of Scer\GAL4Ubi.PU in the hpoBF33 homozygous somatic clones induced in the eye tissue significantly enhances the overgrowth phenotype observed in the adult eye containing hpoBF33 clones.

      (Hu et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      AckKD.Scer\UAS.T:SV5\V5
      AckKD.UAS.Tag:V5
      Name Synonyms
      Secondary FlyBase IDs
        References (1)