FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\lncRNA:CR42858KO
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General Information
Symbol
Dmel\lncRNA:CR42858KO
Species
D. melanogaster
Name
FlyBase ID
FBal0322655
Feature type
allele
Associated gene
Dmel\lncRNA:CR42858
Associated Insertion(s)
TI{TI}lncRNA:CR42858KO
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Wen et al., 2016)
Mutagen
gene targeting by homologous recombination
(Wen et al., 2016)
CRISPR/Cas9
(Wen et al., 2016)
Progenitor genotype
Associated Insertion(s)
TI{TI}lncRNA:CR42858KO
Cytology
Description

The entire lncRNA gene has been knocked out and replaced with an attP site followed by an FRT-flanked Disc\RFP3xP3.cUa fluorescent marker.

(Wen et al., 2016)
Allele components
Component
Use(s)
Also carries
attP
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3R:9,824,085..9,824,864 [-]
Reported_genomic_loc:

3R_r6:9824085..9824864

Comment:

lncRNA region removed by targeted deletion.

(Wen et al., 2016)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      CR42858KO homozygote adults show reduced fertility in males, whereas females are fully fertile compared to wild-type controls and display severe morphological defects in testes (accumulation of abnormal cotton-like flocculus in their distal part) as well as reduced number of mature spermatozoa in the seminal vesicles. They also display defects in spermatid morphogenesis (mutant testes contain sperm cells that are scattered or curled).

      The investment cones of CR42858KO homozygous males are lagging and disorganized during the individualization step of spermatogenesis compared to wild-type.

      (Wen et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      lncRNA:CR42858KO is rescued by lncRNA:CR42858+tWa

      (Wen et al., 2016)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CR42858KO
      lncRNA:CR42858KO
      Name Synonyms
      Secondary FlyBase IDs
        References (1)