Bsg25D^Null/Bsg25D^Null embryos, but not Bsg25D^Null/+ embryos, exhibit a significantly decreased hatching rate, as compared to wild type. Bsg25D^Null/Bsg25D^Null embryos exhibit varying severities of mitotic defects, including excessive nuclear fallout, abnormal nuclear aggregates, bridges between chromosomes, chromosome segregation defects, delocalization of the centrosome from the mitotic spindle, and in some cases failure of cellularization, monopolar and tripolar spindles.

Offspring of Bsg25D^Null/Df(2L)Exel6011 x Bsg25D^Null/Df(2L)Exel6011 crosses exhibit a modest but significant decrease in pole cell number, as compared to controls.

Females expressing Bsg25D^{Scer\UAS.P\T.CR.3'UTR.T:Avic\GFP-EGFP} in a Bsg25D^Null/Df(2L)Exel6011 background do not produce a significantly different number of eggs lacking dorsal appendages, or any significant difference in hatching frequency, as compared to controls.

Females expressing Bsg25D^{Scer\UAS.P\T.CR.T:Avic\GFP-EGFP} in a Bsg25D^Null/Df(2L)Exel6011 background produce a significantly increased number of eggs lacking dorsal appendages, but no difference in hatching frequency, as compared to controls; the dorsal appendage phenotype is not seen when females express Bsg25D^{Scer\UAS.P\T.CR.T:Avic\GFP-EGFP} in a wild type background.