FlyBase Allele Report: Dmel\Strip[RNAi.UAS.9]

General Information

Symbol

Dmel\Strip^RNAi.UAS.9

Species

D. melanogaster

Name

FlyBase ID

FBal0322955

Feature type

allele

Associated gene

Dmel\Strip

Associated Insertion(s)

Carried in Construct

P{UAS-Strip.RNAi.9}

Key Links

Transgenic product class

RNAi_reagent

(Sakuma et al., 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Sakuma et al., 2016)

Mutagen

in vitro construct

(Sakuma et al., 2016)

Progenitor genotype

Carried in construct

P{UAS-Strip.RNAi.9}

Cytology

Description

UAS regulatory sequences drive expression of a shRNA that targets Strip.

(Sakuma et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Strip

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Sakuma et al., 2016)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS

(Sakuma et al., 2016)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Sakuma et al., 2016)

Phenotype Manifest In

actin filament | third instar larval stage, with Scer\GAL4^elav.PU

(Sakuma et al., 2016)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav.PU

(Sakuma et al., 2016)

NMJ bouton | increased number, with Scer\GAL4^RapGAP1-OK6

(Sakuma et al., 2016)

NMJ bouton | increased number, with Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS

(Sakuma et al., 2016)

Detailed Description

Statement

Reference

Expression of Strip^{dsRNA.shRNA.Scer\UAS.9} under the control of Scer\GAL4^RapGAP1-OK6 results in a significant increase in the number of satellite boutons on neuromuscular junctions (NMJ) in third instar larval 6/7 muscles (this increase is also observed in larvae expressing both Strip^{dsRNA.shRNA.Scer\UAS.9} and Strip^{dsRNA.shRNA.Scer\UAS}); the total avg. number of boutons/NMJ as well as the muscle area is unchanged compared to controls. The amplitude of both evoked and spontaneous miniature excitatory junction potentials is unaffected but the frequency of mEJPs is significantly increased in larvae expressing Strip^{dsRNA.shRNA.Scer\UAS.9} compared to controls.

Expression of Strip^{dsRNA.shRNA.Scer\UAS.9} under the control of Scer\GAL4^elav.PU leads to altered actin organization in neuromuscular junctions in third instar larvae.

(Sakuma et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9 has abnormal neuroanatomy | third instar larval stage phenotype, non-enhanceable by Scer\GAL4^RapGAP1-OK6/mts^JF02805

(Sakuma et al., 2016)

Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9 has abnormal neuroanatomy | third instar larval stage phenotype, non-enhanceable by Cka^HM05138, Scer\GAL4^RapGAP1-OK6

(Sakuma et al., 2016)

Suppressed by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by hpo^42-47/hpo[+]

(Sakuma et al., 2016)

Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by wts^x1/wts[+]

(Sakuma et al., 2016)

Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/wts^JF02741

(Sakuma et al., 2016)

Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/ena^S187A.UAS

(Sakuma et al., 2016)

NOT suppressed by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9 has abnormal neurophysiology | third instar larval stage phenotype, non-suppressible by hpo^42-47/hpo[+]

(Sakuma et al., 2016)

Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^RapGAP1-OK6/ena^S187D.UAS

(Sakuma et al., 2016)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9 has NMJ bouton | increased number phenotype, non-enhanceable by Scer\GAL4^RapGAP1-OK6/mts^JF02805

(Sakuma et al., 2016)

Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9 has NMJ bouton | increased number phenotype, non-enhanceable by Cka^HM05138, Scer\GAL4^RapGAP1-OK6

(Sakuma et al., 2016)

Suppressed by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9 has NMJ bouton | increased number phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/wts^JF02741

(Sakuma et al., 2016)

Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9 has NMJ bouton | increased number phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/ena^S187A.UAS

(Sakuma et al., 2016)

Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9 has NMJ bouton | increased number phenotype, suppressible by hpo^42-47/hpo[+]

(Sakuma et al., 2016)

Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9 has NMJ bouton | increased number phenotype, suppressible by wts^x1/wts[+]

(Sakuma et al., 2016)

NOT suppressed by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9 has NMJ bouton | increased number phenotype, non-suppressible by yki^UAS.cHa/Scer\GAL4^RapGAP1-OK6

(Sakuma et al., 2016)

Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9 has NMJ bouton | increased number phenotype, non-suppressible by Scer\GAL4^RapGAP1-OK6/ena^S187D.UAS

(Sakuma et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of satellite boutons on neuromuscular junctions in third instar larvae expressing Strip^{dsRNA.shRNA.Scer\UAS.9} under the control of Scer\GAL4^RapGAP1-OK6 is not further enhanced by co-expression of either mts^JF02805 or Cka^HM05138 but is suppressed by combination with a single copy of wts^x1 or co-expression of wts^JF02741 RNAi, it cannot however be suppressed by co-expression of yki^Scer\UAS.cHa. The satellite bouton number phenotype is rescued by combination with single copy of hpo^42-47, whereas the increased frequency of miniature excitatory junction potentials characteristic for Strip^{dsRNA.shRNA.Scer\UAS.9} expressing larvae remains high. The increased bouton number is also suppressed by co-expression of ena^{S187A.Scer\UAS} but not ena^{S187D.Scer\UAS}.

(Sakuma et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Strip^RNAi.UAS/Strip^RNAi.UAS.9 is rescued by Scer\GAL4^RapGAP1-OK6/Strip^UAS.RR

(Sakuma et al., 2016)

Comments

The increased number of satellite boutons on neuromuscular junctions in third instar larvae simultaneously expressing Strip^{dsRNA.shRNA.Scer\UAS} and Strip^{dsRNA.shRNA.Scer\UAS.9} under the control of Scer\GAL4^RapGAP1-OK6 is rescued by co-expression of Strip^{Scer\UAS.RNAi-res}.

(Sakuma et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Strip^RNAi.UAS.9

Strip^{dsRNA.shRNA.Scer\UAS.9}

Strip^{dsRNA.shRNA.UAS.9}

Name Synonyms

Secondary FlyBase IDs

References (2)

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