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General Information
Symbol
Dmel\Lgr3ag5
Species
D. melanogaster
Name
FlyBase ID
FBal0323559
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A superfolder GFP (sfGFP) coding sequence followed by a flexible linker spacer sequence (GSGSGS) has been inserted into the endogenous Lgr3 locus at the N-terminal end of the Lgr3 open reading frame. The Lgr3 open reading frame is intact and encodes a functional protein.

Insertion components
TI{TI}Lgr3ag5
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

In third instar larvae, Lgr3ag5RA labels approximately 40 cell bodies in the embryonic/larval brain and approximately 140 cell bodies in the ventral nerve cord (VNC). Intense expression was observed in a single pair of neurons at the tip of the VNC, a single dorso-ventral pair of midline neurons in the thoracic segment and a pair of bilateral neurons in the larval pars intercerebralis. The pair of VNC neurons express the cholinergic marker Scer\GAL4ChAT.7.4, the GABAergic marker Scer\GAL4Gad1.3.098 and the glutamatergic marker Scer\GAL4VGlut.PU while the dorso-ventral pair of thoracic midline neurons express Scer\GAL4ChAT.7.4. The neurites of these latter neurons project centrally and then anteriorly towards the brain, where they branch at its base. The bilateral pair of neurons in the larval pars intercerebralis co-express Scer\GAL4ChAT.7.4, Scer\GAL4Gad1.3.098 and Scer\GAL4VGlut.PU. These neurons have a single neurite branching ventrally towards the subesophageal ganglion and dorsally into highly arborized proximal termini which reach nearby insulin producing cells, whose neurites extend through this arborization. These bilateral neurons also associate with axonal projections from Ilp7-XP positive adult dorsomedial neurosecretory cells. A number of other neurons in the larval brain also express Scer\GAL4ChAT.7.4. Approximately 10 neurons per hemisphere co-express Scer\GAL4GMR19B09, including the pair of bilateral neurons in the larval pars intercerebralis. A further 60 or so neurons express Scer\GAL4GMR19B09 in the VNC. The bilateral neurons in the pars intercerebralis also co-express Scer\GAL4Mz699, as do neurons in the embryonic/larval subesophageal ganglion and ventral nerve cord.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Lgr3ag5/Lgr3ag5 mutants do not exhibit any significant delay to pupariation, as compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (1)