FlyBase Allele Report: Dmel\ds[intra.UAS.Tag:V5]

General Information

Symbol

Dmel\ds^{intra.UAS.Tag:V5}

Species

D. melanogaster

Name

FlyBase ID

FBal0324733

Feature type

allele

Associated gene

Dmel\ds

Associated Insertion(s)

Carried in Construct

P{UAS-ds.intra.V5}

Key Links

Transgenic product class

inframe_deletion

(Revilla-Yates et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Revilla-Yates et al., 2015)

Mutagen

in vitro construct

(Revilla-Yates et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-ds.intra.V5}

Cytology

Description

UAS regulatory sequences drive expression of the ds intracellular domain (nucleotides 10153..11464, GenBank:L08811.2). The protein is tagged at the C-terminal end with Tag:V5.

(Revilla-Yates et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tagged with

Tag:V5

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^hh.PU

(Revilla-Yates et al., 2015)

abnormal size | third instar larval stage, with Scer\GAL4^en.PU

(Revilla-Yates et al., 2015)

Phenotype Manifest In

wing blade, with Scer\GAL4^en.PU

(Revilla-Yates et al., 2015)

wing blade posterior compartment, with Scer\GAL4^hh.PU

(Revilla-Yates et al., 2015)

wing disc posterior compartment | third instar larval stage, with Scer\GAL4^en.PU

(Revilla-Yates et al., 2015)

Detailed Description

Statement

Reference

Expressing ds^{intra.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^hh.PU results in smaller relative posterior wing compartment area and smaller anterior-posterior cross-vein distance, as compared to controls.

Expressing ds^{intra.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^en.PU

leads to smaller posterior compartment of third instar larval wing discs without affecting apoptosis, as compared to controls.

Expression of ds^{intra.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^hh.PU in a ds^38k/ds^36D background leads to high lethality during early pupal stages, with only a few adult survivors that show severe wing defects, as compared to ds^38k/ds^36D controls.

(Revilla-Yates et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

ds^{intra.UAS.Tag:V5} fails to rescue ds^38k/ds^D36

(Revilla-Yates et al., 2015)

Comments

Expression of ds^{intra.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^hh.PU fails to rescue the wing defects of ds^38k/ds^36D adults.

(Revilla-Yates et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ds^{intra.Scer\UAS.T:SV5\V5}

ds^{intra.UAS.Tag:V5}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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