FlyBase Allele Report: Hsap\FUS[UAS.cJa.Tag:HA]

General Information

Symbol

Hsap\FUS^{UAS.cJa.Tag:HA}

Species

H. sapiens

Name

FlyBase ID

FBal0324818

Feature type

allele

Associated gene

Hsap\FUS

Associated Insertion(s)

Carried in Construct

P{UAS-HA-hFUS.wt}

Key Links

Transgenic product class

wild_type

(Jäckel et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Jäckel et al., 2015)

Mutagen

in vitro construct

(Jäckel et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-HA-hFUS.wt}

Cytology

Description

UASt regulatory sequences drive expression of wild-type Hsap\FUS that is tagged at the N-terminal end with Tag:HA.

(Jäckel et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\FUS

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis type 6

CEA

(Jäckel et al., 2015)

model of frontotemporal dementia

CEA

(Jäckel et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of amyotrophic lateral sclerosis type 6

is exacerbated by Tnpo^GD33

(Jäckel et al., 2015)

is exacerbated by Tnpo^GD14426

(Jäckel et al., 2015)

is NOT ameliorated by Art1^GD11959

(Jäckel et al., 2015)

is ameliorated by Hsap\TNPO1^UAS.cJa

(Jäckel et al., 2015)

is NOT ameliorated by Art1^KK101196

(Jäckel et al., 2015)

is NOT ameliorated by Art1^JF01306

(Jäckel et al., 2015)

model of frontotemporal dementia

is exacerbated by Tnpo^GD33

(Jäckel et al., 2015)

is NOT ameliorated by Art1^GD11959

(Jäckel et al., 2015)

is exacerbated by Tnpo^GD14426

(Jäckel et al., 2015)

is ameliorated by Hsap\TNPO1^UAS.cJa

(Jäckel et al., 2015)

is NOT ameliorated by Art1^KK101196

(Jäckel et al., 2015)

is NOT ameliorated by Art1^JF01306

(Jäckel et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color, with Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^VGlut1-OK371

(Jäckel et al., 2015)

lethal - all die before end of P-stage, with Scer\GAL4^Toll-6-D42

(Jäckel et al., 2015)

lethal - all die before end of P-stage, with Scer\GAL4^VGlut1-OK371

(Jäckel et al., 2015)

visible, with Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Detailed Description

Statement

Reference

Expression of Hsap\FUS^{Scer\UAS.cJa.T:Ivir\HA1} driven by Scer\GAL4^GMR.PF results in a severe rough eye phenotype, with significant pigment loss, as compared to controls.

Expression of Hsap\FUS^{Scer\UAS.cJa.T:Ivir\HA1} driven by Scer\GAL4^VGlut-OK371 leads to lethality before eclosion and to locomotive defects in third instar larval righting assays, as compared to controls.

Expression of Hsap\FUS^{Scer\UAS.cJa.T:Ivir\HA1} driven by Scer\GAL4^Toll-6-D42 leads to lethality before eclosion.

(Jäckel et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by Tnpo^GD33, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by Tnpo^GD14426, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

NOT Enhanced by

Statement

Reference

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF has visible phenotype, non-enhanceable by Art1^JF01306, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF has abnormal eye color phenotype, non-enhanceable by Art1^JF01306, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF has visible phenotype, non-enhanceable by Art1^GD11959, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF has abnormal eye color phenotype, non-enhanceable by Art1^GD11959, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF has visible phenotype, non-enhanceable by Art1^KK101196, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF has abnormal eye color phenotype, non-enhanceable by Art1^KK101196, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Suppressed by

Statement

Reference

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF has visible phenotype, suppressible | partially by Hsap\TNPO1^UAS.cJa, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF has abnormal eye color phenotype, suppressible | partially by Hsap\TNPO1^UAS.cJa, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF, Tnpo^GD33 has visible phenotype, suppressible | partially by Hsap\TNPO1^UAS.cJa, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF, Tnpo^GD33 has abnormal eye color phenotype, suppressible | partially by Hsap\TNPO1^UAS.cJa, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Tnpo^GD33, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Tnpo^GD14426, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

NOT Enhanced by

Statement

Reference

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by Art1^JF01306, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by Art1^GD11959, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by Art1^KK101196, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Suppressed by

Statement

Reference

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by Hsap\TNPO1^UAS.cJa, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Hsap\FUS^{UAS.cJa.Tag:HA}, Scer\GAL4^GMR.PF, Tnpo^GD33 has eye phenotype, suppressible | partially by Hsap\TNPO1^UAS.cJa, Scer\GAL4^GMR.PF

(Jäckel et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of either Trn^GD33 or Trn^GD14426 strongly enhances the severe eye defects resulting from the expression of Hsap\FUS^{Scer\UAS.cJa.T:Ivir\HA1} driven by Scer\GAL4^GMR.PF, as evident from the appearance of abundant black lesions.

The co-expression of Hsap\TNPO1^Scer\UAS.cJa partially ameliorates the severe eye defects resulting from either the Scer\GAL4^GMR.PF-driven expression of either Hsap\FUS^{Scer\UAS.cJa.T:Ivir\HA1} alone or both Hsap\FUS^{Scer\UAS.cJa.T:Ivir\HA1} and Trn^GD33, as compared to the expression of a control UAS-regulated transgene.

Co-expression of either Art1^GD11959, Art1^KK101196 or Art1^JF01306 does not enhance the severe eye defects resulting from the expression of Hsap\FUS^{Scer\UAS.cJa.T:Ivir\HA1} driven by Scer\GAL4^GMR.PF, as compared to controls.

(Jäckel et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\FUS^{Scer\UAS.cJa.T:Ivir\HA1}

Hsap\FUS^{UAS.cJa.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (1)

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