FB2025_01 , released February 20, 2025
Allele: Dmel\α-Cat421
Open Close
General Information
Symbol
Dmel\α-Cat421
Species
D. melanogaster
Name
FlyBase ID
FBal0325078
Feature type
allele
Associated gene
Dmel\α-Cat
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Jurado et al., 2016)
Progenitor genotype
Cytology
Description

Amino acid replacement: Q459term.

(Jurado et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:23,329,095..23,329,095 [-]
Nucleotide change:

C23329095T

Amino acid change:

Q459term | alpha-Cat-PA

Reported amino acid change:

Q459term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Jurado et al., 2016)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      α-Cat421 mutant embryos show anterior cuticle defects and frequently display holes, some also develop a completely dorsal open phenotype or dorsal holes. The mutants display a reduction in the velocity of progression of the leading edge during dorsal closure compared to wild-type controls, however it is not due to a decrease in the number of cell delamination events as this is actually increased in the mutants and the total number of amnioserosa cells at the onset of tissue contraction is comparable to controls. There are however significant aberrations in the oscillatory and contractile behavior of amnioserosa cells during the dorsal closure process: the period of cell oscillations is increased (the amplitude remains unaffected) as well as the length of both contraction and expansion half-cycles, the rate of apical cell contraction is lower and the cells develop a corrugated appearance. The actin dynamics in these cells is perturbed.

      (Jurado et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      α-Cat421 has embryonic/larval cuticle | embryonic stage phenotype, enhanceable by Df(1)Vinc2

      (Jurado et al., 2016)
      NOT Enhanced by
      Statement
      Reference

      α-Cat421 has embryonic/larval cuticle | embryonic stage phenotype, non-enhanceable by sqhDD.UAS/Scer\GAL4c381

      (Jurado et al., 2016)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The severity of the embryonic cuticle defects characteristic for α-Cat421 mutants is aggravated by combination with Df(1)Vinc2 but not by expression of sqhDD.Scer\UAS under the control of Scer\GAL4c381 in the mutant background.

      (Jurado et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      α-Cat421
      (Jurado et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)