CRISPR/Cas9 mediated non-homologous end joining (NHEJ) resulting in removal of two nucleotides after codon 51 in the 365-residue FoxL1 ORF. The protein from this allele, if made, would encode the first 51 residues of FoxL1 followed by 14 out-of-frame residues, then a stop codon, and does not include the FKH DNA binding domain.
CRISPR/Cas9 mediated removal of two nucleotides in codons 50-51 of the FoxL1 ORF.
FoxL1CR10.5 homozygous mutant embryos do not show any apparent defects in muscle structure and organization. FoxL1CR10.5/FoxL1mCh transheterozygous embryos show high frequency of defects in the salivary gland lumen (including variations in lumen diameter, bending/folding or occasional branching of the lumen) compared to wild-type controls.
