FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Pde4Δ143
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General Information
Symbol
Dmel\Pde4Δ143
Species
D. melanogaster
Name
FlyBase ID
FBal0325387
Feature type
allele
Associated gene
Dmel\Pde4
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Ruppert et al., 2017)
Progenitor genotype
P{EP}Pde4EP973
(Ruppert et al., 2017)
Cytology
Description

Deletion in the first exon of the dnc[RA] transcript (extending from -2148 to +71 relative to the transcription start site of dnc[RA]). This reduces the level of the dnc[RA] transcript almost to zero, slightly reduces dnc[RL] expression and does not affect the expression of the other dnc transcript groups.

(Ruppert et al., 2017)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
X:3,289,096..3,291,314 [+]
Comment:

2219 bp deletion resulting from the imprecise excision of P{EP}dncEP973. The deleton removes sequences from -2148 to +71 relative to the transcription start site of dnc-RA. Deletion was mapped against the dnc-RA tss in R6.16 .

(Ruppert et al., 2017)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Upon initial exposition, dncΔ143 adult mutants show ethanol sensitivity that is comparable to wild-type controls but exhibit reduced development of tolerance (= increase in resistance during re-exposition) as well as decreased heat-induced ethanol resistance normally observed in wild-type flies.

      (Ruppert et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Pde4Δ143 has chemical sensitive | adult stage phenotype, suppressible by hangAE10

      (Ruppert et al., 2017)
      NOT Enhancer of
      Statement
      Reference

      Pde4Δ143 is a non-enhancer of chemical sensitive | adult stage phenotype of hangAE10

      (Ruppert et al., 2017)
      Suppressor of
      Statement
      Reference

      Pde4Δ143 is a suppressor of chemical sensitive | adult stage phenotype of hangAE10

      (Ruppert et al., 2017)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The reduced heat-induced ethanol (EtOH) resistance characteristic for hangAE10 mutant adults is not exacerbated further by combination with dncΔ143 which on its own also lead to a decrease in the heat > ethanol cross-tolerance.

      hangAE10;dncΔ143 double mutant adult flies display normal ethanol tolerance development upon re-exposition to EtOH, unlike each of the single mutants that show significantly reduced tolerance buildout.

      (Ruppert et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Pde4Δ143 is rescued by Scer\GAL4Pde4.RA/Pde4UAS.all

      (Ruppert et al., 2017)

      Pde4Δ143 is rescued by Scer\GAL4Pde4.RA/Pde4UAS.all/Scer\GAL80ple.PK

      (Ruppert et al., 2017)

      Pde4Δ143 is rescued by Pde4UAS.all/Scer\GAL4NP6510

      (Ruppert et al., 2017)

      Pde4Δ143 is rescued by Scer\GAL4Pde4.RA/Pde4UAS.RA.EGFP

      (Ruppert et al., 2017)
      Not rescued by

      Pde4Δ143 is not rescued by Pde4UAS.all/Scer\GAL4GMR58E02

      (Ruppert et al., 2017)

      Pde4Δ143 is not rescued by Scer\GAL4Pde4.RA/Pde4UAS.RG.EGFP

      (Ruppert et al., 2017)

      Pde4Δ143 is not rescued by Pde4UAS.RL.EGFP/Scer\GAL4Pde4.RA

      (Ruppert et al., 2017)
      Comments

      The reduced ability of dncΔ143 mutant adults to develop tolerance to ethanol upon re-exposition is rescued by expression of dncScer\UAS.all when driven by either Scer\GAL4dnc.RA (alone or combined with Scer\GAL80ple.PK to restrict the expression pattern) or Scer\GAL4NP6510 but not when driven by Scer\GAL4GMR58E02. The tolerance buildout can also be rescued by Scer\GAL4dnc.RA-driven expression of dncScer\UAS.RA.T:Avic\GFP-EGFP but not dncScer\UAS.RG.T:Avic\GFP-EGFP or dncScer\UAS.RL.T:Avic\GFP-EGFP.

      (Ruppert et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Pde4Δ143
      dncΔ143
      (Ruppert et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)