CG2021^e43

FlyBase curator comment: The flanking sequence of the P{hsneo}CG2021^neo7 insertion in the 'l(3)neo7' line maps within the CG2021 gene (see FBrf0230166). The cytological location of the insertion inferred from the flanking sequence location is 62B4 (FBrf0230166), which is consistent with the cytological location of 62B determined for the insertion by in situ hybridisation (FBrf0049003) and with the complementation data for the lethality of the line (FBrf0049893, FBrf0111489). However, FBrf0206525 states that the 'l(3)neo7' line affects CG12026 (the annotation corresponding to Tmhs), which is located over 100kB from the site of the P{hsneo}CG2021^neo7 insertion. Tmhs is an ortholog of the human LHFPL5 (or 'TMHS') gene, mutations of which are associated with deafness, and FBrf0206525 shows that heterozygotes of the 'l(3)neo7' line have a defect in the sound-evoked potential in response to an acoustic stimulus. There is no information in FBrf0206525 to help determine whether there is a second insertion, in CG12026, in the 'l(3)neo7' line. For now, this discrepancy is represented in FlyBase by assuming that there are two separable lesions in the 'l(3)neo7' line: 1. the P{hsneo}CG2021^neo7 insertion in the CG2021 gene, to which the lethality of the line (represented by the CG2021^neo7 allele) is assumed to map (as the complementation data is consistent with this interpretation) and 2. a lesion that affects Tmhs and which results in an auditory defect (represented by the Tmhs^P1 allele). ( date:20170227 ).