FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Zzzz\VHHUAS.morphotrap.ext.mCherry
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General Information
Symbol
Zzzz\VHHUAS.morphotrap.ext.mCherry
Species
a. artificial
Name
FlyBase ID
FBal0326385
Feature type
allele
Associated gene
Zzzz\VHH
Associated Insertion(s)
Carried in Construct
M{UAS-morphotrap.ext.mCh}
Also Known As
UAS-morphotrap
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
cre recombinase
(Harmansa et al., 2015)
Progenitor genotype
Zzzz\VHHlexAop.UAS.morphotrap.ext.mCherry
(Harmansa et al., 2015)
Carried in construct
M{UAS-morphotrap.ext.mCh}
Cytology
Description

UAS regulatory sequences and a minimal Hsp70 promoter (derived from the pUASTLOTattB vector) are fused upstream of a fusion protein that consists of the GFP-binding VHH(GFP4) nanobody, the Tag:M(mCd8a) transmembrane domain and the mCherry fluorescent tag. The VHH(GFP4) nanobody is extracellular, allowing it to trap any GFP-tagged extracellular protein, while the mCherry tag is located in the cytosol. (Generated by P1cre-mediated excision of the lexAop regulatory sequences present in Zzzz\vhhGFP4lexAop.UAS.morphotrap.ext.mCherry).

(Harmansa et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
VHH(GFP4)
Tagged with
mCherry
Tag:M(mCd8a)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The wings of dppd8/dppd12 mutants do not develop at all, only rudimentary stubs are formed, this can be rescued by expression of Ecol\lexALG.dpp>dpplexAop.cHa.EGFP : the wings of these adults display venation defects (extra vein material at the anterior crossvein, loss of distal part of L4) and the have slightly elongated shape but their sizes are comparable to that of control wings. The wing size of these rescued flies can in turn be reduced again by hindering the spreading of the dpp morphogen by expressing Zzzz\vhhGFP4Scer\UAS.morphotrap.ext.T:Disc\RFP-mCherry under the control of Scer\GAL4hh.PU (i.e. in the posterior compartment) - the blade size is strongly decreased and the patterning in its posterior part is lost.

      Blocking the dpp spreading in the posterior compartment decreases the posterior pouch size by about 40% in larval wing discs.

      (Harmansa et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Zzzz\VHHUAS.morphotrap.ext.mCherry
      Zzzz\vhhGFP4Scer\UAS.morphotrap.ext.T:Disc\RFP-mCherry
      Zzzz\vhhGFP4UAS.morphotrap.ext.mCherry
      Name Synonyms
      Secondary FlyBase IDs
        References (4)