FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\prg3
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General Information
Symbol
Dmel\prg3
Species
D. melanogaster
Name
FlyBase ID
FBal0326406
Feature type
allele
Associated gene
Dmel\prg
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Nazario-Yepiz and Riesgo-Escovar, 2017)
Progenitor genotype
P{GT1}prgBG02741
(Nazario-Yepiz and Riesgo-Escovar, 2017)
Cytology
Description

Amino acid replacement: D57A.

(Nazario-Yepiz and Riesgo-Escovar, 2017)

Imprecise excision of the progenitor, resulting in point mutations in the promoter region and two missense point mutations in the ORF. The E76D likely does not affect function as a 'D' is found in this position in other Drosophila orthologs.

(Nazario-Yepiz and Riesgo-Escovar, 2017)

Amino acid replacement: E76D.

(Nazario-Yepiz and Riesgo-Escovar, 2017)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:8,464,837..8,464,837 [+]
Nucleotide change:

A8464837C

Amino acid change:

D57A | prg-PA; D57A | prg-PB

Reported amino acid change:

D57A

Comment:

One of two missense mutations in prg coding sequence in mutant. Also has mutations in the promoter region. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Nazario-Yepiz and Riesgo-Escovar, 2017)
point_mutation
2L:8,464,895..8,464,895 [+]
Nucleotide change:

G8464895Y

Amino acid change:

E76D | prg-PA; E76D | prg-PB

Reported amino acid change:

E76D

Comment:

One of two missense mutations in prg coding sequence in mutant. Probably does not affect function since a D residue exists at this position in other Drosophila orthologs. Also has mutations in the promoter region. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Nazario-Yepiz and Riesgo-Escovar, 2017)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      A significant proportion of prg3 homozygote embryos do not develop a cuticle at all and die, leading to decreased hatching rate compared to controls, none of the hatched larvae reach pupation.

      Eggs produced by mosaic mothers with prg3 mutant germline do not develop, are significantly smaller and have abnormal dorsal appendages compared to controls.

      prg3 mutant clones in adult thorax or eye (induced during larval development in the wing and eye imaginal discs) show a number of defects: the thoracic mutant clones lack chaetae and in some cases a slight indentation in the mid-dorsal thorax, the eye clones also lack bristles and show extensive disruption of ommatidial (irregularly spaced and sized ommatidial often with central hole) and retinal architecture (accompanied by frequent pyknotic nuclei and cellular debris) and extreme roughness of the eye surface.

      (Nazario-Yepiz and Riesgo-Escovar, 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The embryonic cuticular defects and high embryonic mortality characteristic for both fweEY08496 and prg3 homozygous mutants are further enhanced by combination with either one or two copies of either prg3 or fweEY08496, respectively.

      (Nazario-Yepiz and Riesgo-Escovar, 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      prg3 is partially rescued by Scer\GAL4Act.PU/prgUAS.cNa

      (Nazario-Yepiz and Riesgo-Escovar, 2017)
      Comments

      Expression of prgScer\UAS.cNa driven by Scer\GAL4Act.PU rescues the embryonic cuticular defects and increased early embryo lethality due to complete lack of cuticle characteristic for prg3 homozygotes but fails to rescue the larval lethality as none of the hatched larvae manage to eclose as adults.

      (Nazario-Yepiz and Riesgo-Escovar, 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      prg3
      Name Synonyms
      Secondary FlyBase IDs
        References (1)